González S, Díaz F, Rius F, Pérez de Vargas I
Department of Dermatology, Faculty of Medicine, University of Malaga, Spain.
J Am Acad Dermatol. 1995 Feb;32(2 Pt 1):218-22. doi: 10.1016/0190-9622(95)90129-9.
In a previous study of 26 patients with psoriasis we analyzed cytophotometrically the nuclear DNA content of the germinative compartment of involved and uninvolved skin by means of the Feulgen technique. These subjects were classified into three groups according to their DNA profile. Group 1 had a monomodal diploid profile, group 2 showed a significantly increased 2C-4C population, and group 3 demonstrated high proportions of 4C and hyperdiploid keratinocytes.
Our purpose was to analyze clinical variables implicated in the development of psoriasis in reference to the three groups.
Nuclear DNA content of each group by quantitative histochemical studies was analyzed and correlated with variables such as chronologic age, sex, age at onset, duration of flare during the study, stress, and the Koebner phenomenon.
No significant differences in DNA profile were observed in the involved epidermis among the clinical variables. The only differences in the uninvolved skin pertained to the duration of the flare, where a statistically significant difference was observed between groups 1 and 3 in the basal (p < or = 0.0459) and suprabasal keratinocytes (p < or = 0.06), and in the Koebner phenomenon, which was induced in all subjects (100%) in groups 2 and 3 and in only 44% of subjects in group 1.
Uninvolved skin of patients with psoriasis should be included in analysis of the clinical behavior of the disease. Furthermore, the Koebner phenomenon is a good clinical indicator of the DNA profile of these subjects.
在之前一项针对26例银屑病患者的研究中，我们采用福尔根技术，通过细胞光度法分析了受累皮肤和未受累皮肤生发层的核DNA含量。这些受试者根据其DNA图谱被分为三组。第1组具有单峰二倍体图谱，第2组显示2C - 4C群体显著增加，第3组表现出高比例的4C和超二倍体角质形成细胞。
我们的目的是参照这三组分析与银屑病发病相关的临床变量。
通过定量组织化学研究分析每组的核DNA含量，并将其与诸如实际年龄、性别、发病年龄、研究期间发作持续时间、压力和同形反应等变量相关联。
在临床变量中，受累表皮的DNA图谱未观察到显著差异。未受累皮肤的唯一差异与发作持续时间有关，在第1组和第3组之间，基底角质形成细胞（p≤0.0459）和基底上层角质形成细胞（p≤0.06）存在统计学显著差异，以及在同形反应方面，第2组和第3组所有受试者（100%）均诱发了同形反应，而第1组只有44%的受试者诱发了同形反应。
银屑病患者未受累皮肤应纳入该疾病临床行为的分析。此外，同形反应是这些受试者DNA图谱的良好临床指标。
