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一种可在自由活动大鼠中多次使用的简易微操纵器:用于电生理学、伏安法和同步脑内注射。

A simple micromanipulator for multiple uses in freely moving rats: electrophysiology, voltammetry, and simultaneous intracerebral infusions.

作者信息

Rebec G V, Langley P E, Pierce R C, Wang Z, Heidenreich B A

机构信息

Department of Psychology, Indiana University, Bloomington 47405.

出版信息

J Neurosci Methods. 1993 Apr;47(1-2):53-9. doi: 10.1016/0165-0270(93)90021-i.

Abstract

An inexpensive, easily fabricated micromanipulator is described that can be used for single-unit recording or voltammetry in freely moving rats. The basic design is configured around the standard coupling system between a plastic syringe and corresponding needle hub. The device can be used with glass or metal microelectrodes for electrophysiology or carbon-fiber or carbon-disk microelectrodes for voltammetry. With either recording technique, the micromanipulator also can accommodate a 33-ga infusion cannula, which allows drugs to be administered directly to the recording site. The entire assembly is lightweight and can be used with a head-mounted amplifier system for relatively noise-free recording.

摘要

本文描述了一种价格低廉、易于制造的微操纵器,可用于自由活动大鼠的单细胞记录或伏安法研究。其基本设计围绕塑料注射器与相应针座之间的标准耦合系统构建。该装置可与玻璃或金属微电极用于电生理学研究,或与碳纤维或碳盘微电极用于伏安法研究。无论采用哪种记录技术,该微操纵器还可容纳一根33号输注套管,从而能够将药物直接注射到记录部位。整个组件重量轻,可与头戴式放大器系统配合使用,以实现相对无噪声的记录。

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