Brasch K, Adams G H, Neelin J M
Cell Tissue Res. 1977 Jan 20;176(3):373-87. doi: 10.1007/BF00221795.
The regenerating blood of geese injected with phenylhydrazine was subjected to large scale, zonal centrifugation through density gradients of Ficoll. In this way, erythroid cells were fractionated according to their respective stages of development. Highly enriched fractions were obtained, containing cells that were well preserved as assessed by both light and electron microscopy. The separated cells exhibited ribosome density and nucleic acid and protein staining patterns typically associated with erythrocyte differentiation. Morphometric analysis of nuclei indicated that despite an apparent net increase in the amount of compact chromatin during development, comparatively little difference existed between the volumes of condensed chromatin present in immature and mature cells. Instead, there was a three fold decrease in nuclear volume between young erythroblasts and reticulocytes, coupled with a concomitant decrease in the volume occupied by dispersed chromatin, RNP and nucleoli. These observations are discussed in relation to molecular changes associated with nuclear differentiation in erythroid cells.
将注射苯肼的鹅的再生血液通过Ficoll密度梯度进行大规模区带离心。通过这种方式，红细胞系细胞根据其各自的发育阶段进行了分级分离。获得了高度富集的级分，其中包含通过光学显微镜和电子显微镜评估均保存良好的细胞。分离出的细胞呈现出通常与红细胞分化相关的核糖体密度以及核酸和蛋白质染色模式。细胞核的形态计量分析表明，尽管在发育过程中紧密染色质的量明显净增加，但未成熟细胞和成熟细胞中存在的凝聚染色质体积之间差异相对较小。相反，幼红细胞和成红细胞之间核体积减少了三倍，同时分散染色质、核糖核蛋白和核仁所占体积也随之减少。结合与红细胞系细胞核分化相关的分子变化对这些观察结果进行了讨论。
