Dardick I, Stterfield G, Wainwright L K
Tissue Cell. 1976;8(2):277-92. doi: 10.1016/0040-8166(76)90052-5.
Light and electron microscopy of suspensions of cells prepared by dispersing chick blastodiscs at primitive-streak and head-fold stages showed the presence of numerous yolk granules, yolk-rich endodermal cells and occasional presumed ecto- and mesodermal cells. Several cell fractions prepared from this suspension by sedimentation through discontinuous Ficoll gradients were of similar composition. No enrichment of any particular cell type which might account for either differential sedimentation or erythropoietic potential of the fractions could be recognized. Two fractions, EP 1, and EP 2, were cultured as cell aggregates on vitelline membranes. EP 1 produced highly organized blood islands containing developing erythrocyte cells, organized endothelium, fibroblasts, thrombocytes and occasional granulocytes. Blood islands derived from EP 2, on the other hand, contained essentially only aggregates of erythroblasts embedded in endoderm. It is tentatively suggested that EP1 contains young multipotential hematopoietic precursors while EP 2 has only older blood-cell precursors committed to erythrocyte development. No cellular basis for the resolution of EP 1 into two complementary subfractions could be recognized.
通过分散处于原条期和头褶期的鸡胚盘制备的细胞悬液，经光镜和电镜观察显示，其中存在大量卵黄颗粒、富含卵黄的内胚层细胞以及偶尔出现的推测为外胚层和中胚层的细胞。通过不连续的菲可梯度沉降从该悬液中制备的几个细胞组分，其组成相似。未发现任何特定细胞类型的富集，而这种富集可能解释各组分的差异沉降或造血潜能。两个组分，即EP 1和EP 2，作为细胞聚集体在卵黄膜上培养。EP 1产生高度组织化的血岛，其中包含正在发育的红细胞、有组织的内皮细胞、成纤维细胞、血小板以及偶尔出现的粒细胞。另一方面，源自EP 2的血岛基本上只包含嵌入内胚层的成红细胞聚集体。初步推测，EP1含有年轻的多能造血前体细胞，而EP 2仅含有已定向于红细胞发育的较老的血细胞前体细胞。未发现EP 1分解为两个互补亚组分的细胞基础。
