Gustafson J M, Burgess E C, Wachal M D, Steinberg H
Department of Medical Sciences, University of Wisconsin-Madison, School of Veterinary Medicine 53706.
Am J Vet Res. 1993 Jun;54(6):882-90.
To determine whether intrauterine transmission of Borrelia burgdorferi could exist in dogs, 10 female Beagles were inoculated intradermally with approximately 1,000 B burgdorferi on day 1 of proestrus; inoculation was repeated every 2 weeks during the gestation period. Ten female control Beagles were similarly inoculated with phosphate-buffered saline solution. Prior to the start of the study, all females and 3 males used for breeding were seronegative for B burgdorferi on the basis of results of the indirect fluorescent antibody test and immunoblot (western analysis. Similarly, results of culture of blood for B burgdorferi were negative. All 20 of the females were bred naturally. Blood samples were collected weekly for serologic testing and culture. Blood samples were obtained from live pups on day 1 of life, then weekly until pups were 6 weeks old when they were euthanatized. Tissues were obtained for culture and testing by use of polymerase chain reaction (PCR). Of 10 spirochete-inoculated (SI) females, 8 became infected with B burgdorferi as evidenced by spirochete culture results and/or PCR-detected B burgdorferi DNA in the tissues of females or their pups. Of the 10 SI females, 8 delivered litters (3 to 7 pups) that had at least 1 neonatal or 6-week-old pup with B burgdorferi DNA-positive tissues (by PCR), and spirochetes were cultured from tissues from pups of 2 litters.(ABSTRACT TRUNCATED AT 250 WORDS)
为确定犬是否存在伯氏疏螺旋体的宫内传播，10只雌性比格犬在发情前期第1天皮内接种约1000个伯氏疏螺旋体；在妊娠期每2周重复接种一次。10只雌性对照比格犬同样接种磷酸盐缓冲盐溶液。在研究开始前，根据间接荧光抗体试验和免疫印迹（western分析）结果，所有用于繁殖的雌性和3只雄性对伯氏疏螺旋体血清学检测均为阴性。同样，血液培养伯氏疏螺旋体的结果为阴性。所有20只雌性均自然交配。每周采集血样进行血清学检测和培养。在幼犬出生第1天采集血样，然后每周采集一次，直至幼犬6周龄时实施安乐死。获取组织进行培养，并采用聚合酶链反应（PCR）检测。在10只接种螺旋体（SI）的雌性中，8只感染了伯氏疏螺旋体，雌性或其幼犬组织中的螺旋体培养结果和/或PCR检测到的伯氏疏螺旋体DNA可证明这一点。在10只SI雌性中，8只产下了幼崽（3至7只幼犬），其中至少有1只新生幼犬或6周龄幼犬的组织经PCR检测伯氏疏螺旋体DNA呈阳性，并且从2窝幼犬的组织中培养出了螺旋体。（摘要截选至250字）
