Knight J A, Searles D A, Blaylock R C
Pathology and Laboratory Medicine Service, Salt Lake VA Medical Center, UT.
Ann Clin Lab Sci. 1993 May-Jun;23(3):178-83.
Since the introduction of acid-citrate-dextrose (ACD) in 1947 to anticoagulate and preserve whole blood for transfusion, various improved formulas, such as citrate-phosphate-dextrose-adenine (CPDA-1), have been successfully introduced, extending the current acceptable blood storage time to 35 days. Additional nutrient-anticoagulant solutions, including CPDA-2, CPDA-3, AS-1, and AS-5, among others, have more recently been studied with comparable results. In this study, lipid peroxidation (LP) was compared, as determined by the measurement of plasma malondialdehyde (MDA) by liquid chromatography, in stored blood preserved with various nutrient solutions with blood treated with CPDA-1. Although minor but inconsistent differences were noted with most of these solutions compared with CPDA-1, AS-1 gave consistently lower MDA levels (P < 0.01). Further improvements in red cell storage and viability may require approaches other than nutrient formula variations, such as the addition of metal chelating agents or other selected antioxidants to the nutrient-anticoagulant solutions, donor supplementation with vitamin free radical scavengers, or other as yet undetermined conditions.
自1947年引入酸-枸橼酸盐-葡萄糖（ACD）用于抗凝和保存全血以供输血以来，各种改进配方已成功推出，如枸橼酸盐-磷酸盐-葡萄糖-腺嘌呤（CPDA-1），将目前可接受的血液储存时间延长至35天。其他营养抗凝溶液，包括CPDA-2、CPDA-3、AS-1和AS-5等，最近也进行了研究，结果相当。在本研究中，通过液相色谱法测定血浆丙二醛（MDA）来比较脂质过氧化（LP），比较了用各种营养溶液保存的血液与用CPDA-1处理的血液。尽管与CPDA-1相比，大多数这些溶液存在微小但不一致的差异，但AS-1的MDA水平始终较低（P < 0.01）。红细胞储存和活力的进一步改善可能需要营养配方变化以外的方法，如在营养抗凝溶液中添加金属螯合剂或其他选定的抗氧化剂、供体补充维生素自由基清除剂或其他尚未确定的条件。
