Knight J A, Searles D A, Clayton F C
Salt Lake VA Medical Center, UT, USA.
Ann Clin Lab Sci. 1996 Jul-Aug;26(4):283-90.
Acid-citrate-dextrose (ACD) was introduced in 1947 as a nutrient-anticoagulant solution to preserve stored whole blood. A decade later, the introduction of citrate-phosphate-dextrose (CPD) resulted in the successful preservation of whole blood for 21 days. In 1978, added adenine and increased glucose concentration (CPDA-1) increased storage to 35 days. More recently, other solutions such as AS-1, further increased the acceptable storage time of packed cells, but cost and inconvenience have limited their use. Several recent studies have investigated the possibility of increasing the shelf-life of whole blood by the addition of various antioxidants. In the current study a comparison was made of the effects of the metal chelator desferrioxamine on several parameters of stored red cells. Thus, measurement was made of plasma malondialdehyde levels, a measure of lipid peroxidation, red cell filtration times, a measure of red cell deformability, and red cell morphology by electron microscopy. Our results showed that adding desferrioxamine significantly decreased levels of plasma malondialdehyde (MDA) and slowed the loss of erythrocyte deformability. Although red cell morphology suggested that the treated cells maintained their normal appearance longer than untreated cells, the differences were not statistically significant. These results suggest that the addition of desferrioxamine to CPDA-1 anticoagulated blood might be effective in prolonging the viability of stored erythrocytes.
1947年引入了酸性枸橼酸盐葡萄糖（ACD）作为一种营养抗凝溶液来保存库存全血。十年后，枸橼酸盐磷酸盐葡萄糖（CPD）的引入使得全血成功保存21天。1978年，添加腺嘌呤并提高葡萄糖浓度（CPDA-1）使保存时间延长至35天。最近，诸如AS-1等其他溶液进一步延长了浓缩红细胞的可接受保存时间，但成本和不便限制了它们的使用。最近的几项研究探讨了通过添加各种抗氧化剂来延长全血保质期的可能性。在本研究中，对金属螯合剂去铁胺对储存红细胞的几个参数的影响进行了比较。因此，测量了血浆丙二醛水平（衡量脂质过氧化的指标）、红细胞过滤时间（衡量红细胞变形性的指标）以及通过电子显微镜观察红细胞形态。我们的结果表明，添加去铁胺可显著降低血浆丙二醛（MDA）水平，并减缓红细胞变形性的丧失。尽管红细胞形态显示处理后的细胞比未处理的细胞更长时间保持正常外观，但差异无统计学意义。这些结果表明，在CPDA-1抗凝血液中添加去铁胺可能有效地延长储存红细胞的活力。
