通过原生质体融合进行基因操作以及产黄青霉生产青霉素。
Gene manipulation by protoplast fusion and penicillin production by Penicillium chrysogenum.
作者信息
Tahoun M K
机构信息
Department of Agricultural Industries, Faculty of Agriculture, University of Alexandria, Egypt.
出版信息
Appl Biochem Biotechnol. 1993 Spring;39-40:445-53. doi: 10.1007/BF02919009.
Hybrids have been obtained by protoplast fusion of nitrate-nonutilizing cnx- and acetate-nonutilizing fac- mutants of Penicillium chrysogenum strains 4/95 and 26/818, respectively. Induced haplodization of the hybrids allowed the recovery of stable segregants, which were screened for penicillin production. The penicillin-producing segregants showed a wide range of titers that reached for a certain mutant to 290-390% increase above the parent strains.
通过分别对产黄青霉4/95菌株的硝酸盐非利用型cnx -突变体和26/818菌株的乙酸盐非利用型fac -突变体进行原生质体融合，获得了杂种。杂种的诱导单倍体化使得能够获得稳定的分离株，并对其青霉素生产情况进行筛选。产青霉素的分离株显示出广泛的效价范围，对于某一突变体而言，其效价比亲本菌株提高了290 - 390%。
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