Oda T, Kanai Y, Shimoyama Y, Nagafuchi A, Tsukita S, Hirohashi S
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):897-904. doi: 10.1006/bbrc.1993.1710.
Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis. In this report, we present the human alpha-catenin sequence. Human alpha-catenin showed extensive homology with that of mouse, i.e., 91.8% and 99.3% at the nucleic acid and amino acid levels, respectively, indicating that this molecule has been evolutionarily conserved in mammals. Characterization of the mRNA sequence of alpha-catenin in PC9 was also carried out, and two distinct abnormal sequences, i.e., one of 957 bp deletion resulting in a 319-amino-acid deletion and another of 761 bp deletion resulting in a frameshift, were identified. These deletions were probably produced by an error of RNA splicing, presenting one possible mechanism for the loss of intact alpha-catenin expression.
钙黏蛋白和连环蛋白构成细胞黏附复合体，对紧密的细胞间黏附至关重要。这种黏附复合体功能失调会导致癌细胞从原发性肿瘤结节中解离，从而可能促进癌症侵袭和转移。在本报告中，我们展示了人类α-连环蛋白序列。人类α-连环蛋白与小鼠的α-连环蛋白具有广泛的同源性，即在核酸水平和氨基酸水平上分别为91.8%和99.3%，这表明该分子在哺乳动物中具有进化保守性。我们还对PC9中α-连环蛋白的mRNA序列进行了表征，鉴定出两个不同的异常序列，即一个957 bp的缺失导致319个氨基酸的缺失，另一个761 bp的缺失导致移码。这些缺失可能是由RNA剪接错误产生的，这是完整α-连环蛋白表达缺失的一种可能机制。
