钡、锶和钙在化合物48/80诱导的组胺释放中的相互作用。

Interaction between barium, strontium and calcium in histamine release by compound 48/80.

作者信息

Payne A N, Garland L G

出版信息

Eur J Pharmacol. 1978 Dec 1;52(3-4):329-34. doi: 10.1016/0014-2999(78)90286-8.

Abstract

Pre-incubating rat peritoneal and pleural mast cells for 30 min at 2 degrees C in Ca2+-free Tyrode containing Ba2+ (30 mM) markedly reduced histamine release by compound 48/80 (0.5 microgram/ml). Evoked secretion was not significantly altered by pre-incubation with Sr2+ (30 mM) at this temperature whilst Mg2+ (30 mM) and La3+ (0.1 mM) slightly inhibited and potentiated release respectively. The effect of Ba2+ was concentration related and progressively reversed by Ca2+ (1--10 mM). Pre-incubation at 37 degrees C in Ca2+-free medium alone substantially reduced histamine release by compound 48/80. This reduction was unaltered by either Mg2+ (30 mM) or La3+ (0.1 mM), prevented by either Sr2+ (30 mM) or Ca2+ (1 mM) and augmented by Ba2+ (30 mM) which also markedly increased spontaneous histamine release during pre-incubation at 37 degrees C. Results suggest that Ba2+ and Sr2+ interact with cell-fixed calcium to modulate histamine release by compound 48/80.

摘要

将大鼠腹膜和胸膜肥大细胞在含钡离子（30 mM）的无钙台氏液中于2℃预孵育30分钟，可显著降低化合物48/80（0.5微克/毫升）诱导的组胺释放。在此温度下，用锶离子（30 mM）预孵育对诱发的分泌无显著影响，而镁离子（30 mM）和镧离子（0.1 mM）分别轻微抑制和增强释放。钡离子的作用与浓度相关，可被钙离子（1 - 10 mM）逐渐逆转。仅在无钙培养基中于37℃预孵育可显著降低化合物48/80诱导的组胺释放。这种降低不受镁离子（30 mM）或镧离子（0.1 mM）影响，可被锶离子（30 mM）或钙离子（1 mM）阻止，且钡离子（30 mM）可增强这种降低作用，同时钡离子还显著增加了37℃预孵育期间的自发性组胺释放。结果表明，钡离子和锶离子与细胞固定钙相互作用，以调节化合物48/80诱导的组胺释放。