Alm P E, Bloom G D
Int Arch Allergy Appl Immunol. 1981;66(4):427-38. doi: 10.1159/000232851.
The effect of norepinephrine on transmembrane passage of calcium in rat peritoneal mast cells, was studied in an in vitro system. It was found the histamine release from mast cells induced by the ionophore A 23 187 in normal calcium medium and compound 48/80 in a calcium-free medium was suppressed by 10(-3) M norepinephrine but not at concentrations in the range 10(-5)-10(-4) M. When the secretory process is totally dependent on the presence of calcium in the incubation medium, i.e. calcium-depleted cells, 10(-5) M norepinephrine suppresses the histamine release induced by low concentrations of compound 48/80. The effect of norepinephrine (10(-5) M) on strontium-induced "spontaneous' histamine release was also studied. It was found that norepinephrine (10(-5) M) totally inhibits the progressive histamine release induced by strontium. It is possible to evoke secretion in a calcium-free medium, and subsequent introduction of Ca2+ will result in optimal histamine release. This demonstrates a secretory process in which we can distinguish between utilization of endogenous versus exogenous calcium. The release that is dependent on extracellular calcium is inhibited by norepinephrine (10(-5) M). These data indicate that the suppressive effect of norepinephrine (concentrations less than 10(-3) M) on histamine release from rat mast cells is due to an interference in transmembrane passage of calcium.
在体外系统中研究了去甲肾上腺素对大鼠腹膜肥大细胞钙跨膜转运的影响。发现在正常钙培养基中离子载体A 23187和无钙培养基中化合物48/80诱导的肥大细胞组胺释放受到10⁻³M去甲肾上腺素的抑制，但在10⁻⁵ - 10⁻⁴M范围内的浓度时则无此作用。当分泌过程完全依赖于孵育培养基中钙的存在时，即钙耗尽的细胞，10⁻⁵M去甲肾上腺素可抑制低浓度化合物48/80诱导的组胺释放。还研究了去甲肾上腺素（10⁻⁵M）对锶诱导的“自发性”组胺释放的影响。发现去甲肾上腺素（10⁻⁵M）完全抑制锶诱导的组胺渐进性释放。在无钙培养基中可以引发分泌，随后引入Ca²⁺将导致最佳组胺释放。这表明了一种分泌过程，在其中我们可以区分内源性钙与外源性钙的利用。依赖于细胞外钙的释放受到去甲肾上腺素（10⁻⁵M）的抑制。这些数据表明去甲肾上腺素（浓度小于10⁻³M）对大鼠肥大细胞组胺释放的抑制作用是由于对钙跨膜转运的干扰。
