Rehak N N, Janes G, Young D S
Clin Chem. 1977 Feb;23(2 PT. 1):195-9.
Uric acid in serum was determined calorimetrically with a batch type microcalorimeter, by measuring the heat evolved during a coupled uricase/catalase enzymic reaction in tris(hydroxymethyl)aminomethane HCl buffer (pH 9.0 at 30 degrees C). Heat evolution and concentration are linearly related through the physiological range of serum uric acid concentrations and the method is free of interferences of the sort encountered with spectrophotometric methods. Precision and accuracy are good (CV, 2%) and the results correlate well with those obtained by a mechanized colorimetric uricase/peroxidase system.
采用批式微量量热计通过测量在三（羟甲基）氨基甲烷盐酸盐缓冲液（30℃时pH 9.0）中尿酸酶/过氧化氢酶偶联酶促反应过程中释放的热量，以量热法测定血清中的尿酸。在血清尿酸浓度的生理范围内，热量释放与浓度呈线性关系，并且该方法不存在分光光度法所遇到的那种干扰。精密度和准确度良好（变异系数为2%），并且结果与通过机械化比色尿酸酶/过氧化物酶系统获得的结果相关性良好。
