遗传性高血压中血管平滑肌细胞对血小板衍生生长因子反应性的年龄依赖性改变。

Age-dependent alterations in vascular smooth muscle cell responsiveness to platelet-derived growth factor in genetic hypertension.

Saltis J, Agrotis A, Bobik A

Baker Medical Research Institute, Alfred Hospital, Prahran, Victoria, Australia.

Clin Exp Pharmacol Physiol. 1993 May;20(5):324-6. doi: 10.1111/j.1440-1681.1993.tb01695.x.

Abstract

This study examined and compared the growth characteristics of vascular smooth muscle cells (VSMC) isolated from 4 and 12 week old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats following stimulation with platelet-derived growth factor-BB (PDGF-BB). 2. Vascular smooth muscle cells from 4 week old SHR proliferated at a slower rate than VSMC isolated from 12 week old SHR (1.08 +/- 0.06/day vs 1.89 +/- 0.13/day respectively, P < 0.05). In contrast, there was no difference in the proliferation of VSMC from 4 and 12 week WKY rats (0.62 +/- 0.06/day vs 0.82 +/- 0.13/day, respectively, P > 0.05). 3. The cell density at which VSMC from 4 and 12 week old SHR become refractory to the mitogenic effects of PDGF-BB was similar and approximately two-fold greater than VSMC from age-matched WKY rats. 4. This study concludes that there is an age-dependent, differential and specific upregulation of growth rate mechanisms in VSMC from SHR which enhance proliferation in response to PDGF-BB.

摘要

本研究检测并比较了从4周龄和12周龄自发性高血压大鼠（SHR）以及Wistar-Kyoto（WKY）大鼠分离出的血管平滑肌细胞（VSMC）在血小板衍生生长因子-BB（PDGF-BB）刺激后的生长特性。2. 4周龄SHR的血管平滑肌细胞增殖速度比12周龄SHR分离出的VSMC慢（分别为1.08±0.06/天和1.89±0.13/天，P<0.05）。相比之下，4周龄和12周龄WKY大鼠的VSMC增殖无差异（分别为0.62±0.06/天和0.82±0.13/天，P>0.05）。3. 4周龄和12周龄SHR的VSMC对PDGF-BB促有丝分裂作用产生不应性时的细胞密度相似，且比同龄WKY大鼠的VSMC约高两倍。4. 本研究得出结论，SHR的VSMC中存在与年龄相关的、差异性和特异性的生长速率机制上调，这增强了其对PDGF-BB的增殖反应。