血小板衍生生长因子A链同二聚体对自发性高血压大鼠培养的平滑肌细胞的特异性生长刺激作用。
Specific growth stimulation of cultured smooth muscle cells from spontaneously hypertensive rats by platelet-derived growth factor A-chain homodimer.
作者信息
Resink T J, Scott-Burden T, Hahn A W, Rouge M, Hosang M, Powell J S, Bühler F R
机构信息
Department of Research, Basel University Hospital, Switzerland.
出版信息
Cell Regul. 1990 Oct;1(11):821-31. doi: 10.1091/mbc.1.11.821.
Cultured vascular smooth muscle cells (VSMC)1 from spontaneously hypertensive rats (SHR) possess specific cell surface receptors for both homodimeric forms of platelet-derived growth factor (PDGF-AA and PDGF-BB), in contrast to cells from normotensive Wistar Kyoto (WKY) animals, which express receptors only for the B-chain form of PDGF. Stimulation of quiescent VSMC from SHR with PDGF-AA resulted in activation of S6-kinase and induction of phosphoinositide catabolism, as well as cellular proliferation when cultures were maintained for prolonged periods with daily supplementation of the growth factor. WKY-derived VSMC showed no response to PDGF-AA, which was consistent with their lack of specific receptors for this homodimer. The responsiveness of quiescent cells from SHR and WKY to the B-chain homodimer was similar. The enhanced growth responsiveness of SHR-derived cells to fetal calf serum, as compared with cells from their normotensive counterparts, may be accounted for in part by their expression of receptors for the AA homodimer of PDGF.
与正常血压的Wistar Kyoto（WKY）动物的细胞不同，自发性高血压大鼠（SHR）的培养血管平滑肌细胞（VSMC）1具有血小板衍生生长因子（PDGF-AA和PDGF-BB）两种同型二聚体形式的特异性细胞表面受体，WKY动物的细胞仅表达PDGF B链形式的受体。用PDGF-AA刺激SHR的静止VSMC会导致S6激酶激活、磷酸肌醇分解代谢诱导，并且当每天补充生长因子将培养物长期维持时会导致细胞增殖。源自WKY的VSMC对PDGF-AA无反应，这与其缺乏这种同型二聚体的特异性受体一致。SHR和WKY静止细胞对B链同型二聚体的反应性相似。与正常血压对应动物的细胞相比，SHR衍生细胞对胎牛血清的生长反应性增强，这可能部分归因于它们对PDGF AA同型二聚体受体的表达。
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