Ibarz E, Palmgren M G, Palazón J, Piñol M T, Serrano R
European Molecular Biology Laboratory, Heidelberg, Germany.
Biochim Biophys Acta. 1994 Nov 23;1196(1):93-6. doi: 10.1016/0005-2736(94)90300-x.
Plasma membrane vesicles were purified from tobacco callii and the modulation of H(+)-ATPase by detergents was investigated. The nonionic detergent Brij 58 not only activated ATP hydrolysis (2-fold) but also proton pumping (more than 4-fold). Triton X-100, within a more limited concentration range, produced a similar effect. The simultaneous activation of ATP hydrolysis and proton pumping is not compatible with current interpretations of effects of nonionic detergents on the H(+)-ATPase based on latency of the enzyme and opening of vesicles.
从烟草愈伤组织中纯化出质膜囊泡，并研究了去污剂对H(+)-ATP酶的调节作用。非离子去污剂Brij 58不仅能激活ATP水解（2倍），还能激活质子泵（超过4倍）。Triton X-100在更有限的浓度范围内也产生了类似的效果。ATP水解和质子泵的同时激活与目前基于酶的潜伏性和囊泡开放对非离子去污剂对H(+)-ATP酶作用的解释不相符。
