Mischak H, Goodnight J, Henderson D W, Osada S, Ohno S, Mushinski J F
Molecular Genetics Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
FEBS Lett. 1993 Jul 12;326(1-3):51-5. doi: 10.1016/0014-5793(93)81759-s.
A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-theta (PKC-theta) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-theta mRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)+ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines.
利用逆转录和聚合酶链反应（PCR），从小鼠骨骼肌mRNA中克隆出一个2.2kb的cDNA，它包含小鼠蛋白激酶C-θ（PKC-θ）的完整编码区。该克隆用作探针，研究这种PKC同工型在正常和转化造血细胞及其他正常组织中的表达。在来自睾丸的聚腺苷酸加尾（poly(A)+）RNA的Northern印迹上，发现PKC-θ mRNA的最高稳态水平是一个2.8kb的转录本。在骨骼肌、脾脏、T淋巴瘤和纯化的正常T淋巴细胞中也发现了高水平，但这些组织和细胞表达两种转录本，分别为3.3kb和3.8kb。在正常脑、B淋巴细胞、B淋巴细胞肿瘤和细胞系中发现了较低水平的类似大小的转录本。
