Ning Y, Lovell M, Cooley L D, Pereira-Smith O M
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030.
Genomics. 1993 Jun;16(3):758-60. doi: 10.1006/geno.1993.1260.
Following fusion of human diploid fibroblast-derived microcells with mouse A9 cells, we isolated seven monochromosomal hybrids containing a single human chromosome 2, 3, 5, 12, 15, 20, or 22. Cytogenetic analysis as well as PCR karyotyping (chromosome-specific banding pattern generated by Alu-PCR) was performed on all the hybrids. We here present, for the first time, the specific PCR karyotypes of human chromosomes 2 and 20.
将源自人二倍体成纤维细胞的微细胞与小鼠A9细胞融合后，我们分离出了七个单染色体杂种细胞，它们分别含有一条人类2号、3号、5号、12号、15号、20号或22号染色体。对所有杂种细胞进行了细胞遗传学分析以及PCR核型分析（通过Alu-PCR产生的染色体特异性条带模式）。我们首次在此展示了人类2号和20号染色体的特异性PCR核型。
