人二倍体成纤维细胞来源的微细胞与小鼠A9细胞融合后单染色体杂种的分离。
Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells.
作者信息
Ning Y, Lovell M, Taylor L, Pereira-Smith O M
机构信息
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030.
出版信息
Cytogenet Cell Genet. 1992;60(1):79-80. doi: 10.1159/000133300.
PMID:1582264
Abstract
Rodent x human hybrids containing single human chromosomes are valuable tools in the study of human genetics. Using a retrovirus carrying the neomycin gene, we generated a neomycin-resistant (neor) pool of human diploid fibroblasts. Following fusion of microcells prepared from this pool to mouse A9 cells, we obtained four neor hybrids containing a single human chromosome 2, 3, 5, or 15.
摘要
含有单条人类染色体的啮齿动物-人类杂种细胞是人类遗传学研究中的宝贵工具。利用携带新霉素基因的逆转录病毒，我们构建了一个对新霉素具有抗性（neor）的人类二倍体成纤维细胞库。将从该细胞库制备的微细胞与小鼠A9细胞融合后，我们获得了四个分别含有单条人类2号、3号、5号或15号染色体的neor杂种细胞。
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