Ohshima M, Otsuka K, Kuwata F, Fujikawa K, Ito K, Suzuki K
Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan.
J Nihon Univ Sch Dent. 1993 Mar;35(1):57-64. doi: 10.2334/josnusd1959.35.57.
To examine whether the gingival epithelium provokes a loss of connective tissue attachment during periodontitis, periodontal ligament (PDL) cells and gingival fibroblasts (GF) were cultured with conditioned medium of gingival epithelial cells, and the collagenolytic activity of PDL cells and GF were examined, respectively. The epithelial cells were cultured in the presence or absence of lipopolysaccharide (LPS), and the respective conditioned media were added to the cultures of PDL cells and GF. The collagenolytic response of PDL cells to both of the conditioned media was 3- to 10-fold higher than that of each control, whereas the response of GF was only 1.4-fold higher. The LPS-stimulated epithelial conditioned medium showed a stimulation rate for collagenolytic activity similar to that of the LPS-free conditioned medium. These results suggest that human gingival epithelial cells, without LPS stimulation, secrete a substance which accelerates the collagenolytic enzyme activity of PDL cells for degradation of PDL fibers, in the absence of GF enzyme activity.
为了研究在牙周炎期间牙龈上皮是否会引发结缔组织附着丧失，将牙周膜（PDL）细胞和牙龈成纤维细胞（GF）与牙龈上皮细胞的条件培养基一起培养，并分别检测PDL细胞和GF的胶原olytic活性。上皮细胞在有或无脂多糖（LPS）的情况下培养，然后将各自的条件培养基添加到PDL细胞和GF的培养物中。PDL细胞对两种条件培养基的胶原olytic反应比每个对照组高3至10倍，而GF的反应仅高1.4倍。LPS刺激的上皮条件培养基显示出与无LPS条件培养基相似的胶原olytic活性刺激率。这些结果表明，在没有GF酶活性的情况下，未经LPS刺激的人牙龈上皮细胞会分泌一种物质，该物质会加速PDL细胞的胶原olytic酶活性，以降解PDL纤维。
