Terranova V P, Nishimura F
Laboratory of Tumor Biology and Connective Tissue Research, Bronx VAMC, New York 10468, USA.
J Dent Res. 1996 Apr;75(4):993-1001. doi: 10.1177/00220345960750041501.
Periodontal ligament (PDL) cell motility and the passage of PDL cells along a root surface are important components of tissue remodeling during periodontal regeneration. Proteolytic enzymes, including fibroblast collagenase, have been demonstrated to play an important role in tissue remodeling. Previous studies have shown that PDL cells chemotactically respond to a variety of matrix and growth factors. We therefore studied the effects of type I collagen fragments and fibroblast collagenase on PDL cell migration, since PDL cells have been shown to adhere preferentially to partially demineralized root surfaces with exposed type I collagen. Gingival epithelial cells were used as a control cell population. We report that PDL cells but not gingival epithelial cells preferentially migrate in a dose-dependent manner to both fibroblast collagenase and to type I collagen degradation products. Epithelial cell migration to fibroblast collagenase and type I collagen fragments was observed. Antibody to type I collagen inhibited the type I collagen fragment-mediated migration. Collagenase pre-treatment of PDL cells enhanced PDL cell migration to type I collagen fragments. In other assays, enzyme inhibitors were shown to decrease the collagenase-mediated PDL cell motility. Epithelial cells were shown to migrate preferentially to 92-kDa type IV collagenase and type IV collagen degradation products. Antibody to type IV collagen inhibited type IV collagen-induced epithelial cell migration. Taken together, these data suggest a role for collagenase in the fine control of PDL cell migration in tissue remodeling during periodontal regeneration.
牙周膜（PDL）细胞的运动性以及PDL细胞沿牙根表面的移动是牙周再生过程中组织重塑的重要组成部分。包括成纤维细胞胶原酶在内的蛋白水解酶已被证明在组织重塑中起重要作用。先前的研究表明，PDL细胞对多种基质和生长因子具有趋化反应。因此，我们研究了I型胶原片段和成纤维细胞胶原酶对PDL细胞迁移的影响，因为已表明PDL细胞优先粘附于暴露有I型胶原的部分脱矿牙根表面。牙龈上皮细胞用作对照细胞群体。我们报告称，PDL细胞而非牙龈上皮细胞以剂量依赖性方式优先向成纤维细胞胶原酶和I型胶原降解产物迁移。观察到上皮细胞向成纤维细胞胶原酶和I型胶原片段的迁移。I型胶原抗体抑制了I型胶原片段介导的迁移。对PDL细胞进行胶原酶预处理可增强PDL细胞向I型胶原片段的迁移。在其他试验中，酶抑制剂可降低胶原酶介导的PDL细胞运动性。上皮细胞被证明优先向92-kDa IV型胶原酶和IV型胶原降解产物迁移。IV型胶原抗体抑制IV型胶原诱导的上皮细胞迁移。综上所述，这些数据表明胶原酶在牙周再生过程中组织重塑时对PDL细胞迁移的精细调控中发挥作用。
