Alexander A A, Liu J B, McCue P, Gomella L G, Ross R P, Lattime E C
Department of Radiology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania.
J Urol. 1993 Aug;150(2 Pt 1):525-8. doi: 10.1016/s0022-5347(17)35540-4.
Experimental studies in the therapy of intravesically growing bladder tumors in mice have been hampered by an inability to monitor tumor growth before and during treatment. To establish a repeatable, noninvasive method to monitor the intravesical growth of bladder tumors, MB49 murine bladder tumor cells were instilled into the bladders of syngeneic C57BL/6 mice. Following 3 weeks of growth, the bladders of tumor-bearing and control mice were imaged using a 20 mHz, 6.2 F catheter-based ultrasound transducer inserted rectally. Bladders of tumor implanted and control mice were identified by high resolution endoluminal ultrasound after distension with 0.15 ml. of normal saline. When compared with the results of histologic analysis, transrectal ultrasound (TRUS) accurately identified tumor presence, size, and location.
由于无法在治疗前和治疗期间监测小鼠膀胱内生长的膀胱肿瘤的生长情况，针对此类肿瘤治疗的实验研究受到了阻碍。为建立一种可重复的、非侵入性的方法来监测膀胱肿瘤在膀胱内的生长情况，将MB49小鼠膀胱肿瘤细胞注入同基因C57BL/6小鼠的膀胱中。生长3周后，使用经直肠插入的20兆赫兹、6.2F基于导管的超声换能器对荷瘤小鼠和对照小鼠的膀胱进行成像。在用0.15毫升生理盐水扩张后，通过高分辨率腔内超声识别植入肿瘤的小鼠和对照小鼠的膀胱。与组织学分析结果相比，经直肠超声（TRUS）能准确识别肿瘤的存在、大小和位置。
