Johnson C D, Stretton A O
J Neurosci. 1987 Jan;7(1):223-35. doi: 10.1523/JNEUROSCI.07-01-00223.1987.
We have used GABA-specific antisera to detect GABA-immunoreactivity in the motor neurons of the ventral nerve cord of Ascaris. We find that a subset of the individually identifiable commissures of motor neurons is specifically stained. On the basis of the location and morphology of stained commissures and of the location of stained cell bodies in the ventral nerve cord, we conclude that the labeled neurons comprise all members of the VI (inhibiting ventral muscle; 13 cells) and DI (inhibiting dorsal muscle; 6 cells) classes of inhibitory motor neurons (Stretton et al., 1978; Walrond et al., 1985). This result supports previous suggestions (e.g., del Castillo et al., 1964b) that GABA is the neurotransmitter released by the inhibitory motor neurons of nematodes. In the anterior part of the animal, the inhibitory motor neuron commissures have small branches in the sublateral nerve cords that have not been previously described: VI commissures have dorsal sublateral branches, while DI cells have ventral branches. Posterior VI neurons have branches in the lateral nerve cords.
我们使用γ-氨基丁酸（GABA）特异性抗血清来检测蛔虫腹神经索运动神经元中的GABA免疫反应性。我们发现运动神经元中可单独识别的连合纤维的一个子集被特异性染色。根据染色连合纤维的位置和形态以及腹神经索中染色细胞体的位置，我们得出结论，标记的神经元包括VI类（抑制腹侧肌肉；13个细胞）和DI类（抑制背侧肌肉；6个细胞）抑制性运动神经元的所有成员（斯特雷顿等人，1978年；沃尔隆德等人，1985年）。这一结果支持了先前的推测（例如，德尔卡斯蒂略等人，1964年b），即GABA是线虫抑制性运动神经元释放的神经递质。在动物的前部，抑制性运动神经元连合纤维在外侧神经索中有小分支，这是以前未描述过的：VI类连合纤维有背外侧分支，而DI类细胞有腹侧分支。后部的VI类神经元在外侧神经索中有分支。
