体外低密度脂蛋白的过氧化物酶依赖性非金属氧化：体内氧化的模型？
Peroxidase-dependent metal-independent oxidation of low density lipoprotein in vitro: a model for in vivo oxidation?
作者信息
Wieland E, Parthasarathy S, Steinberg D
机构信息
Department of Medicine, University of California, San Diego, La Jolla 92093-0682.
出版信息
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5929-33. doi: 10.1073/pnas.90.13.5929.
Abstract
Oxidative modification of low density lipoprotein is believed to be an important pathway by which the lipoprotein becomes atherogenic. The in vitro systems for oxidative modification of low density lipoprotein thus far described all appear to depend upon the presence in the medium of free transition metal ions (copper or iron). In vivo, on the other hand, these metals are present almost exclusively in tightly complexed forms that do not catalyze oxidative modification. The present studies describe oxidation of low density lipoprotein in a simple system that does not depend upon the presence of added free metal ions. It requires the presence of horseradish peroxidase and either hydrogen peroxide or lipid hydroperoxides.
摘要
低密度脂蛋白的氧化修饰被认为是脂蛋白变得具有致动脉粥样硬化性的一条重要途径。迄今为止所描述的用于低密度脂蛋白氧化修饰的体外系统似乎都依赖于培养基中游离过渡金属离子（铜或铁）的存在。另一方面，在体内，这些金属几乎完全以紧密络合的形式存在，不会催化氧化修饰。本研究描述了在一个不依赖于添加游离金属离子的简单系统中低密度脂蛋白的氧化。它需要辣根过氧化物酶以及过氧化氢或脂质氢过氧化物的存在。
相似文献
1
Peroxidase-dependent metal-independent oxidation of low density lipoprotein in vitro: a model for in vivo oxidation?体外低密度脂蛋白的过氧化物酶依赖性非金属氧化：体内氧化的模型？
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5929-33. doi: 10.1073/pnas.90.13.5929.
2
Oxidative modification of human low-density lipoprotein by horseradish peroxidase in the absence of hydrogen peroxide.辣根过氧化物酶在无过氧化氢情况下对人低密度脂蛋白的氧化修饰。
Free Radic Res. 1998 Nov;29(5):427-34. doi: 10.1080/10715769800300471.
3
Oxidation of heparin-treated low density lipoprotein by peroxidases.过氧化物酶对肝素处理的低密度脂蛋白的氧化作用。
Atherosclerosis. 1999 Oct;146(2):211-9. doi: 10.1016/s0021-9150(99)00127-6.
4
5
Pro-oxidant effects of lipoxygenase-derived peroxides on the copper-initiated oxidation of low-density lipoprotein.脂氧合酶衍生的过氧化物对铜引发的低密度脂蛋白氧化的促氧化作用。
Biochem J. 1992 Mar 15;282 ( Pt 3)(Pt 3):631-4. doi: 10.1042/bj2820631.
6
Linoleic acid intake and susceptibility of very-low-density and low density lipoproteins to oxidation in men.男性体内亚油酸摄入量与极低密度脂蛋白和低密度脂蛋白氧化易感性的关系
Am J Clin Nutr. 1996 May;63(5):698-703. doi: 10.1093/ajcn/63.5.698.
7
A critical overview of the chemistry of copper-dependent low density lipoprotein oxidation: roles of lipid hydroperoxides, alpha-tocopherol, thiols, and ceruloplasmin.铜依赖性低密度脂蛋白氧化化学的批判性综述：脂质氢过氧化物、α-生育酚、硫醇和铜蓝蛋白的作用。
Arch Biochem Biophys. 2001 Oct 1;394(1):117-35. doi: 10.1006/abbi.2001.2509.
8
Nitric oxide inhibition of lipoxygenase-dependent liposome and low-density lipoprotein oxidation: termination of radical chain propagation reactions and formation of nitrogen-containing oxidized lipid derivatives.一氧化氮对脂氧合酶依赖性脂质体和低密度脂蛋白氧化的抑制作用：自由基链传播反应的终止及含氮氧化脂质衍生物的形成。
Arch Biochem Biophys. 1995 Dec 1;324(1):15-25. doi: 10.1006/abbi.1995.9935.
9
On-line EPR study of free radicals induced by peroxidase/H(2)O(2) in human low-density lipoprotein.人低密度脂蛋白中过氧化物酶/H₂O₂诱导产生的自由基的在线电子顺磁共振研究
Biochim Biophys Acta. 2002 Jul 11;1583(2):176-84. doi: 10.1016/s1388-1981(02)00211-1.
10
Effect of plasma on the degradation of hydroperoxides of unesterified linoleic acid and copper-peroxidized LDL.血浆对未酯化亚油酸和铜过氧化低密度脂蛋白氢过氧化物降解的影响。
Free Radic Biol Med. 1994 Apr;16(4):459-63. doi: 10.1016/0891-5849(94)90123-6.
引用本文的文献
1
Endoscopically injectable and self-crosslinkable hydrogel-mediated stem cell transplantation for alleviating esophageal stricture after endoscopic submucosal dissection.内镜可注射且可自交联水凝胶介导的干细胞移植用于减轻内镜黏膜下剥离术后食管狭窄
Bioeng Transl Med. 2023 Apr 18;8(3):e10521. doi: 10.1002/btm2.10521. eCollection 2023 May.
2
oxLDL and eLDL Induced Membrane Microdomains in Human Macrophages.氧化低密度脂蛋白和乙酰化低密度脂蛋白诱导人巨噬细胞中的膜微区。
PLoS One. 2016 Nov 21;11(11):e0166798. doi: 10.1371/journal.pone.0166798. eCollection 2016.
3
Impact of Oxidative Stress in Premature Aging and Iron Overload in Hemodialysis Patients.氧化应激对血液透析患者早衰和铁过载的影响。
Oxid Med Cell Longev. 2016;2016:1578235. doi: 10.1155/2016/1578235. Epub 2016 Oct 5.
4
Enzymatically modified low-density lipoprotein is recognized by c1q and activates the classical complement pathway.酶修饰的低密度脂蛋白可被C1q识别并激活经典补体途径。
J Lipids. 2011;2011:376092. doi: 10.1155/2011/376092. Epub 2011 Mar 9.
5
Oxidized low-density lipoprotein.氧化型低密度脂蛋白
Methods Mol Biol. 2010;610:403-17. doi: 10.1007/978-1-60327-029-8_24.
6
Lipid peroxidation and decomposition--conflicting roles in plaque vulnerability and stability.脂质过氧化与分解——在斑块易损性和稳定性中相互矛盾的作用。
Biochim Biophys Acta. 2008 May;1781(5):221-31. doi: 10.1016/j.bbalip.2008.03.002. Epub 2008 Mar 25.
7
Identification of the prooxidant site of human ceruloplasmin: a model for oxidative damage by copper bound to protein surfaces.人铜蓝蛋白促氧化剂位点的鉴定：蛋白质表面结合铜导致氧化损伤的模型
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11546-51. doi: 10.1073/pnas.94.21.11546.
8
Oxidation of low-density lipoprotein by hypochlorite causes aggregation that is mediated by modification of lysine residues rather than lipid oxidation.次氯酸盐对低密度脂蛋白的氧化会导致聚集，这种聚集是由赖氨酸残基的修饰而非脂质氧化介导的。
Biochem J. 1994 Aug 15;302 ( Pt 1)(Pt 1):297-304. doi: 10.1042/bj3020297.
9
Paradoxical actions of antioxidants in the oxidation of low density lipoprotein by peroxidases.过氧化物酶催化低密度脂蛋白氧化过程中抗氧化剂的矛盾作用。
J Clin Invest. 1995 Jun;95(6):2594-600. doi: 10.1172/JCI117961.
10
On the pathogenesis of atherosclerosis: enzymatic transformation of human low density lipoprotein to an atherogenic moiety.关于动脉粥样硬化的发病机制：人类低密度脂蛋白向致动脉粥样硬化部分的酶促转化。
J Exp Med. 1995 Dec 1;182(6):1959-71. doi: 10.1084/jem.182.6.1959.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.人血清中超离心分离的脂蛋白的分布及化学组成
J Clin Invest. 1955 Sep;34(9):1345-53. doi: 10.1172/JCI103182.
3
Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids.内皮细胞对低密度脂蛋白的修饰涉及脂质过氧化和低密度脂蛋白磷脂的降解。
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3883-7. doi: 10.1073/pnas.81.12.3883.
4
5
Macrophage oxidation of low density lipoprotein generates a modified form recognized by the scavenger receptor.巨噬细胞对低密度脂蛋白的氧化作用会产生一种可被清道夫受体识别的修饰形式。
Arteriosclerosis. 1986 Sep-Oct;6(5):505-10. doi: 10.1161/01.atv.6.5.505.
6
Antiatherogenic effect of probucol unrelated to its hypocholesterolemic effect: evidence that antioxidants in vivo can selectively inhibit low density lipoprotein degradation in macrophage-rich fatty streaks and slow the progression of atherosclerosis in the Watanabe heritable hyperlipidemic rabbit.普罗布考的抗动脉粥样硬化作用与其降胆固醇作用无关：体内抗氧化剂可选择性抑制富含巨噬细胞的脂肪条纹中低密度脂蛋白降解并减缓渡边遗传性高脂血症兔动脉粥样硬化进展的证据。
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7725-9. doi: 10.1073/pnas.84.21.7725.
7
Probucol prevents the progression of atherosclerosis in Watanabe heritable hyperlipidemic rabbit, an animal model for familial hypercholesterolemia.普罗布考可阻止渡边遗传性高脂血症兔（一种家族性高胆固醇血症动物模型）动脉粥样硬化的进展。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5928-31. doi: 10.1073/pnas.84.16.5928.
8
Nonenzymatic oxidative cleavage of peptide bonds in apoprotein B-100.载脂蛋白B-100中肽键的非酶促氧化裂解
J Lipid Res. 1987 Dec;28(12):1466-77.
9
10
Hydroperoxide-dependent cooxidation of 13-cis-retinoic acid by prostaglandin H synthase.前列腺素H合酶对13-顺式视黄酸的氢过氧化物依赖性共氧化作用。
J Biol Chem. 1987 Oct 15;262(29):14119-33.
Zotero 插件