Wieland E, Parthasarathy S, Steinberg D
Department of Medicine, University of California, San Diego, La Jolla 92093-0682.
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5929-33. doi: 10.1073/pnas.90.13.5929.
Oxidative modification of low density lipoprotein is believed to be an important pathway by which the lipoprotein becomes atherogenic. The in vitro systems for oxidative modification of low density lipoprotein thus far described all appear to depend upon the presence in the medium of free transition metal ions (copper or iron). In vivo, on the other hand, these metals are present almost exclusively in tightly complexed forms that do not catalyze oxidative modification. The present studies describe oxidation of low density lipoprotein in a simple system that does not depend upon the presence of added free metal ions. It requires the presence of horseradish peroxidase and either hydrogen peroxide or lipid hydroperoxides.
低密度脂蛋白的氧化修饰被认为是脂蛋白变得具有致动脉粥样硬化性的一条重要途径。迄今为止所描述的用于低密度脂蛋白氧化修饰的体外系统似乎都依赖于培养基中游离过渡金属离子（铜或铁）的存在。另一方面，在体内，这些金属几乎完全以紧密络合的形式存在，不会催化氧化修饰。本研究描述了在一个不依赖于添加游离金属离子的简单系统中低密度脂蛋白的氧化。它需要辣根过氧化物酶以及过氧化氢或脂质氢过氧化物的存在。
