Parthasarathy Sampath, Raghavamenon Achuthan, Garelnabi Mahdi Omar, Santanam Nalini
Division of Cardiothoracic Surgery, The Ohio State University Medical Center, Columbus, OH, USA.
Methods Mol Biol. 2010;610:403-17. doi: 10.1007/978-1-60327-029-8_24.
Oxidized low-density lipoprotein (Ox-LDL) has been studied for over 25 years. Numerous pro- and anti-atherogenic properties have been attributed to Ox-LDL. Yet, Ox-LDL has neither been defined nor characterized, as its components and composition change depending on its source, method of preparation, storage, and use. It contains unoxidized and oxidized fatty acid derivatives both in the ester and free forms, their decomposition products, cholesterol and its oxidized products, proteins with oxidized amino acids and cross-links, and polypeptides with varying extents of covalent modification with lipid oxidation products, and many others. It seems to exist in vivo in some form not yet fully characterized. Until its pathophysiological significance, and how it is generated in vivo are determined, the nature of its true identity will be only of classical interest. In this review, its components, their biological actions and methods of preparation will be discussed.
氧化型低密度脂蛋白（Ox-LDL）已被研究了25年以上。Ox-LDL具有许多促动脉粥样硬化和抗动脉粥样硬化特性。然而，Ox-LDL尚未得到明确的定义和表征，因为其成分和组成会因其来源、制备方法、储存和使用方式而有所变化。它包含酯形式和游离形式的未氧化和氧化脂肪酸衍生物、它们的分解产物、胆固醇及其氧化产物、含有氧化氨基酸和交联的蛋白质，以及与脂质氧化产物具有不同程度共价修饰的多肽等等。它似乎以某种尚未完全表征的形式存在于体内。在其病理生理学意义以及它在体内如何产生被确定之前，其真实身份的本质将仅具有传统意义上的研究价值。在这篇综述中，将讨论其成分、生物学作用和制备方法。
