Ernst L K, Duchemin A M, Anderson C L
Department of Internal Medicine, Ohio State University, Columbus 43210.
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6023-7. doi: 10.1073/pnas.90.13.6023.
How receptors for the Fc portion of IgG antibodies (Fc gamma R) trigger a variety of immune functions by clustering upon engagement of ligand is largely unknown. Of the three distinct classes of human Fc gamma R, only Fc gamma RIIIA has been shown to associate with a potential signal-generating subunit, either the gamma chain or the zeta chain. With these studies we show that Fc gamma RI, the high-affinity receptor for IgG, also is found in association with gamma chain. This association can be demonstrated by using anti-Fc gamma RI antibodies, Fc gamma RI ligand, and anti-gamma-chain antibodies that coadsorb the proteins from detergent lysates of Fc gamma RI-expressing cells. The association of Fc gamma RI and gamma chain can be reconstituted by cotransfection of cDNAs for both proteins into COS cells.
IgG抗体Fc段受体（FcγR）如何通过配体结合时的聚集来触发多种免疫功能，目前很大程度上尚不清楚。在人类三种不同类型的FcγR中，只有FcγRIIIA已被证明与潜在的信号产生亚基相关联，即γ链或ζ链。通过这些研究我们表明，IgG的高亲和力受体FcγRI也与γ链相关联。这种关联可以通过使用抗FcγRI抗体、FcγRI配体以及能从表达FcγRI的细胞的去污剂裂解物中共吸附这些蛋白质的抗γ链抗体来证明。FcγRI和γ链的关联可以通过将两种蛋白质的cDNA共转染到COS细胞中进行重建。
