Betterle C, Fabris F, Burul A, Bersani T, Girolami A
Folia Haematol Int Mag Klin Morphol Blutforsch. 1978;105(4):539-43.
Rabbit raised anti-alpha-1-antitrypsin or anti alpha-2-macroglobulin antisera at dilution of less than 1:80 yielded non-specific staining on human platelets by indirect immunofluorescent technique. A similar pattern was in fact obtained by using normal rabbit sera at the same dilution and was due to the presence of smooth muscle autoantibodies. This indicates that human platelets do not contain significant quantities of these antigens. In agreement with the above, only microamounts of alpha-1-antitrypsin and alpha-2-macroglobulin were found to be present in human platelets by means of the electroimmunoassay.
用间接免疫荧光技术检测时，稀释度低于1:80的兔抗α-1-抗胰蛋白酶或抗α-2-巨球蛋白抗血清会在人血小板上产生非特异性染色。事实上，使用相同稀释度的正常兔血清也会得到类似结果，这是由于存在平滑肌自身抗体。这表明人血小板中不含有大量这些抗原。与上述情况一致，通过电免疫测定法发现人血小板中仅存在微量的α-1-抗胰蛋白酶和α-2-巨球蛋白。
