Plotsky Y, Cahaner A, Haberfeld A, Lavi U, Lamont S J, Hillel J
Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Anim Genet. 1993 Apr;24(2):105-10. doi: 10.1111/j.1365-2052.1993.tb00249.x.
An efficient approach to detect association between quantitative traits and bands of DNA fingerprint patterns uses intra-family tail analysis, which compares fingerprints of DNA mixes from individuals at the two tails of a phenotypic distribution. In analysis of 67 paternal half-sibs of a meat-type chicken family, of 57 sire bands generated by two probes, one sire-specific band (S6.6) was associated with abdominal fat deposition. The band effect was estimated by a linear model analysis to be 0.88 standard deviations, or about 30% of the family mean. The association between band S6.6 and abdominal fat was further examined by testing progeny of paternal half-sibs of the chickens which were used in the tail analysis, establishing genetic linkage between the DNA marker and a genetic locus affecting abdominal fat deposition.
一种检测数量性状与DNA指纹图谱条带之间关联的有效方法是采用家系内尾部分析，该方法比较表型分布两端个体的DNA混合物指纹。在对一个肉用型鸡家族的67个父系半同胞进行分析时，由两个探针产生的57个父系条带中，有一个父系特异性条带（S6.6）与腹部脂肪沉积有关。通过线性模型分析估计该条带效应为0.88个标准差，约为家族均值的30%。通过对尾部分析中所用鸡的父系半同胞后代进行检测，进一步研究了条带S6.6与腹部脂肪之间的关联，从而确定了DNA标记与影响腹部脂肪沉积的基因座之间的遗传连锁关系。
