Zieliński K, Walasek G, Werka T, Wesierska M, Gradkowska M, Oderfeld-Nowak B
Department of Neurophysiology, Nencki Institute of Experimental Biology, Warsaw, Poland.
Behav Brain Res. 1993 May 31;55(1):77-84. doi: 10.1016/0166-4328(93)90009-f.
Acquisition of the conditioned emotional response (CER) in 32 male hooded rats previously learned to press a bar for food and divided into four groups was studied. Two groups received electrolytic lesions of the dorsal hippocampal afferent and were thereafter injected either with GM1 ganglioside (30 mg/kg daily) or with buffer. Two remaining groups were sham operated and similarly injected. The partial hippocampal deafferentation evoked immediate enhancement of bar presses rate which persisted during the 2-week period of testing. CER training undertaken 2 days after surgical procedures appeared unsuccessful, whereas similar training with a cue of different modality initiated a week later resulted in acquisition of conditioned suppression of bar presses in all groups. Toward the end of training the conditioned suppression was more pronounced in lesioned than in control rats. The GM1 injections attenuated the conditioned suppression in control rats, presumably due to an antinociceptive role of ganglioside treatment. Behavioural training did not change the normal distribution pattern in cholinergic and serotonergic hippocampal afferent markers showing dorso-ventral gradient along longitudinal axis. The lesion-induced decrease pattern was also not affected. However, in contrast to previous findings in non-trained animals, the GM1 treatment was not effective in protecting against degenerative changes in the hippocampus of trained rats.
对32只雄性带帽大鼠进行了研究，这些大鼠此前已学会按压杠杆获取食物，并被分为四组。其中两组接受了背侧海马传入纤维的电解损伤，之后分别注射GM1神经节苷脂（每日30mg/kg）或缓冲液。其余两组进行假手术并同样注射。部分海马传入纤维切断立即引起杠杆按压率增加，这一现象在为期2周的测试期间持续存在。手术2天后进行的条件性情绪反应（CER）训练似乎未成功，而在一周后开始的使用不同形式线索的类似训练，使所有组均获得了杠杆按压的条件性抑制。在训练接近尾声时，损伤组大鼠的条件性抑制比对照组更为明显。GM1注射减弱了对照组大鼠的条件性抑制，这可能是由于神经节苷脂治疗的抗伤害感受作用。行为训练并未改变胆碱能和血清素能海马传入标记物沿纵轴的背腹梯度的正常分布模式。损伤引起的减少模式也未受影响。然而，与之前对未训练动物的研究结果不同，GM1治疗对防止训练大鼠海马的退行性变化无效。
