Selective isolation of highly polymorphic (dC-dA)n.(dG-dT)n microsatellites by stringent hybridization.
作者信息
Iizuka M, Makino R, Sekiya T, Hayashi K
机构信息
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
出版信息
Genet Anal Tech Appl. 1993 Feb;10(1):2-5. doi: 10.1016/1050-3862(93)90017-d.
PMID:8329220
Abstract
We defined a hybridization condition for isolation of clones carrying long (dC-dA)n.(dG-dT)n microsatellites from a genomic plasmid library. By using (dC-dA)20 oligonucleotide as a probe and hybridizing under stringent conditions, more than two-thirds of the obtained plasmid carried the repeats that were longer than (dC-dA)14 and highly polymorphic.
摘要
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