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Characterization of canine microsatellites.

作者信息

Holmes N G, Humphreys S J, Binns M M, Curtis R, Holliman A, Scott A M

机构信息

Department of Immunogenetics, Animal Health Trust, Newmarket, UK.

出版信息

EXS. 1993;67:415-20. doi: 10.1007/978-3-0348-8583-6_41.

Abstract

Canine DNA was cloned in M13 and screened for the presence of (dC-dA)n.(dG-dT)n repeats. Oligonucleotide primers were synthesised to the microsatellite flanking sequences and used in the polymerase chain reaction to amplify those loci from genomic DNA. The polymorphism of each microsatellite was estimated in a set of unrelated dogs.

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