Takiya C M, Guerret S, Porto L C, Grimaud J A
Universidade Federal do Rio de Janiero, Hospital Universitário Clementino Fraga Filho-Departamento de Patologia, Brasil.
Exp Toxicol Pathol. 1993 May;45(2-3):121-8. doi: 10.1016/S0940-2993(11)80483-5.
In vivo degradation of the extracellular matrix of Schistosoma mansoni granulomas was morphologically studied by light and electron microscopy using 60 day-infected hepatic isolated and acellular granulomas (aPMC) which had been implanted into the subcutaneous tissue of syngenic albino mice and examined 4, 15, 20, 30 and 40 days after implantation. The progressive changes of aPMCs were estimated by measurements of aPMCs surface (micron2) on paraffin sections. After implantation, polymorphonuclear leukocytes were the initial population recruited whereas macrophages and fibroblasts were prominent in the late stages. Eosinophil leukocytes and in a lesser extent, mast cells increased in number. In electron microscopy, progressive disappearance of extracellular matrix and some modified collagen fibrils either in extracellular or intracellular localization represented matricial changes. The morphometrical analysis of aPMCs of the surface (micron2) after implantation showed a significant reduction of aPMCs (p < 0.001) in all stages after implantation. Polymorphonuclear cells, macrophages and fibroblasts seemed to be the major responsible cells involved in aPMCs resorption.
利用60日龄感染曼氏血吸虫的肝脏分离无细胞肉芽肿（aPMC），将其植入同基因白化小鼠的皮下组织，并在植入后4、15、20、30和40天进行检查，通过光学显微镜和电子显微镜从形态学上研究曼氏血吸虫肉芽肿细胞外基质的体内降解情况。通过测量石蜡切片上aPMC的表面积（平方微米）来评估aPMC的渐进性变化。植入后，多形核白细胞是最初募集的细胞群体，而巨噬细胞和成纤维细胞在后期较为突出。嗜酸性粒细胞以及数量较少的肥大细胞数量增加。在电子显微镜下，细胞外基质的逐渐消失以及细胞外或细胞内定位的一些改性胶原纤维代表了基质变化。植入后对aPMC表面积（平方微米）的形态计量分析显示，植入后所有阶段aPMC均显著减少（p < 0.001）。多形核细胞、巨噬细胞和成纤维细胞似乎是参与aPMC吸收的主要责任细胞。
