Andrade Z A, Medeiros M V, Azevedo T M, Guerret S
Centro de Pesquisas Gonçalo Moniz, FIOCRUZ/UFBa, Salvador, BA, Brasil.
Mem Inst Oswaldo Cruz. 1990 Jan-Mar;85(1):79-85. doi: 10.1590/s0074-02761990000100013.
Amorphous material and altered collagen fragments within dilated secretory vesicles and cisternae of fibroblast cytoplasm were the main ultrastructural changes seen in hepatic periovular granulomas formed in mice infected with Schistosoma mansoni and treated with colchicine. Despite promoting ultrastructural changes in the fibroblasts found in hepatic periovular granulomas, colchicine administration to infected mice did not significantly change the light microscopic appearance of the hepatic schistosomal lesions, did not diminish the amount of total hepatic collagen, and did not change the collagen isotypes in the granulomas, as observed after a comparative study with non-colchicine treated infected control mice. When administered to mice two weeks after curative treatment of schistosomiasis with praziquantel, colchicine did not seem to increase extracellular collagen degradation or to induce a more rapid resorption of hepatic periovular granulomas, although still promoting ultrastructural alterations in fibroblasts.
在感染曼氏血吸虫并用秋水仙碱治疗的小鼠肝脏卵周肉芽肿中，成纤维细胞胞质扩张的分泌囊泡和潴泡内的无定形物质及改变的胶原片段是主要的超微结构变化。尽管秋水仙碱给药会促使肝脏卵周肉芽肿中的成纤维细胞发生超微结构变化，但与未用秋水仙碱治疗的感染对照小鼠进行比较研究后发现，给感染小鼠施用秋水仙碱并未显著改变肝脏血吸虫病病变的光学显微镜外观，未减少肝脏总胶原量，也未改变肉芽肿中的胶原同型。在用吡喹酮对血吸虫病进行根治性治疗两周后给小鼠施用秋水仙碱，秋水仙碱似乎并未增加细胞外胶原降解或诱导肝脏卵周肉芽肿更快吸收，尽管它仍会促使成纤维细胞发生超微结构改变。
