Boloukhere M, Baldo-Correa E, Borojevic R
Department of Molecular Biology, Free University of Brussels, Belgium.
J Submicrosc Cytol Pathol. 1993 Oct;25(4):505-17.
Connective tissue cells present in periovular granulomas, induced in mice livers by schistosomal infection, were studied in situ and in an in vitro culture after cell migration from explanted granulomas. They were compared to cells of the adjacent hepatic tissue. Connective tissue cells in granulomas and granuloma-derived primary cell lines were characterized as myofibroblasts. The presence of lipid droplets in cells of early granulomas, and comparison with adjacent perisinusoidal cells, indicated their origin through activation and mobilization of lipocytes from the adjacent hepatic tissue. This origin was confirmed in long-standing cultures of granuloma-derived cells, in the stationary phase of growth, in which myofibroblasts could return spontaneously into the fat-storing phenotype.
对血吸虫感染诱导的小鼠肝脏卵周肉芽肿中的结缔组织细胞进行了原位研究，并在细胞从植入的肉芽肿迁移后进行了体外培养研究。将它们与相邻肝组织的细胞进行了比较。肉芽肿和肉芽肿衍生的原代细胞系中的结缔组织细胞被鉴定为肌成纤维细胞。早期肉芽肿细胞中脂滴的存在以及与相邻窦周细胞的比较表明，它们起源于相邻肝组织中脂肪细胞的激活和动员。在肉芽肿衍生细胞的长期培养中，即在生长的静止期，肌成纤维细胞可自发恢复为储存脂肪的表型，这证实了这种起源。
