Heining P, Hoffmann R W
Institute of Zoology and Hydrobiology, University of Munich, Germany.
Exp Toxicol Pathol. 1993 May;45(2-3):167-76. doi: 10.1016/S0940-2993(11)80499-9.
Trichloroethylene, an organochlorine compound used as solvent in numerous industrial processes, was studied with regard to its effects on rainbow trout Oncorhynchus mykiss. Fish were exposed to trichloroethylene via the surrounding water in sublethal concentrations (0.5; 2.5 mg/l) for a period of 21 or 28 days. Three different commercial products of trichloroethylene, all highly purified but varying in quality and amount of stabilizing agents were used. Subchronic exposure resulted in forced apoptosis as well as adaptive and degenerative changes at the subcellular and cellular level in gills, liver, spleen, head- and trunk kidney at the lower concentration. At the higher concentration, necrotic lesions mainly in liver, spleen and head-kidney were found. Trichloroethylene showed particular affinity to the haematopoietic tissue with proliferation and elevated phagocytic activity of reticulo-endothelial cells. Moreover, an increase in phagocytosis of red blood cells which showed abnormalities in ultrastructure was striking. The histopathologic changes after exposure to three different commercial products of trichloroethylene varying in content of stabilizing agents did not show distinct variations.
