Fahmy F S, Navsaria H A, Frame J D, Jones C R, Leigh I M
North East Thomes Regional Plastic and Burn Unit, St Andrew's Hospital, Billericay, UK.
Br J Plast Surg. 1993 Jun;46(4):292-5. doi: 10.1016/0007-1226(93)90005-v.
The viability of human split skin grafts stored in four solutions has been assessed by monitoring the percentage of viable keratinocytes in the stored grafts. Skin grafts stored in RM+ (Ready Mix) tissue culture medium remained more viable than those stored in Hartmann's, Marshall's or saline solutions. By day 10 (postoperative), the percentage of viable keratinocytes of those grafts stored in RM+ was around 85%, compared to a value of around 10% for the other media. By day 30, RM+ achieved a value of around 60% keratinocyte viability compared to a value approaching 1% in the other storage media under investigation. RM+ provides mitogens, nutrients, growth factors and physiological pH, all of which are important factors for successful skin graft storage.
通过监测储存移植物中活角质形成细胞的百分比，评估了储存在四种溶液中的人断层皮片的活力。储存在RM+（即用型）组织培养基中的皮肤移植物比储存在哈特曼氏溶液、马歇尔氏溶液或盐溶液中的移植物更具活力。到术后第10天，储存在RM+中的那些移植物的活角质形成细胞百分比约为85%，而其他培养基中的该值约为10%。到第30天，RM+实现了约60%的角质形成细胞活力，而在所研究的其他储存培养基中该值接近1%。RM+提供有丝分裂原、营养物质、生长因子和生理pH值，所有这些都是成功储存皮肤移植物的重要因素。
