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一步法与两步法硫代胆碱铜法用于胆碱酯酶细胞化学定位的比较。

A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases.

作者信息

Klinar B, Brzin M

出版信息

Histochemistry. 1977 Feb 1;50(4):313-8. doi: 10.1007/BF00507124.

DOI:10.1007/BF00507124
PMID:833016
Abstract

A comparison between the one-step and the two step copper thiocholine procedure for the subcellular localization of cholinesterases was undertaken. The results indicate that only under experimental conditions with short incubation times and precise control of the conversion into sulphide is the localization of the primary precipitate and that of the secondary precipitate identical. It was concluded that the conversion of the primary precipitate into Cu-sulphide is not necessary and can lead to artefacts.

摘要

对硫代胆碱铜一步法和两步法用于胆碱酯酶亚细胞定位进行了比较。结果表明，只有在孵育时间短且能精确控制转化为硫化物的实验条件下，初级沉淀物和次级沉淀物的定位才相同。得出的结论是，将初级沉淀物转化为硫化铜没有必要，且可能会导致人为假象。

相似文献

1
A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases.一步法与两步法硫代胆碱铜法用于胆碱酯酶细胞化学定位的比较。
Histochemistry. 1977 Feb 1;50(4):313-8. doi: 10.1007/BF00507124.
2
Iodide, thiocyanate and cyanide ions as capturing reagents in one-step copper-thiocholine method for cytochemical localization of cholinesterase activity.在一步铜硫代胆碱法中，碘离子、硫氰酸根离子和氰离子作为捕获试剂用于胆碱酯酶活性的细胞化学定位。
Histochemistry. 1976 Sep 13;48(4):283-92. doi: 10.1007/BF00499245.
3
Quantitative evaluation of the trapping reaction of copperthiocholine histochemical procedures for localization of cholinesterases.用于胆碱酯酶定位的硫代胆碱铜组织化学方法捕获反应的定量评估。
Histochemistry. 1978 Jul 12;56(3-4):213-20. doi: 10.1007/BF00495982.
4
Electron microscopic localization of cholinesterase by a copper-lead-thiocholine technique.用铜-铅-硫代胆碱技术对胆碱酯酶进行电子显微镜定位
J Neurochem. 1966 Dec;13(12):1345-9. doi: 10.1111/j.1471-4159.1966.tb04297.x.
5
A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.一种用于胆碱酯酶的“直接显色”硫代胆碱法。
J Histochem Cytochem. 1964 Mar;12:219-21. doi: 10.1177/12.3.219.
6
Ultrastructural localization of acetylcholinesterase activity by means of the electron dense precipitate derived from Koelle's cuprous thiocholine iodide by treatment with phosphomolybdic acid and osmium tetroxide.通过用磷钼酸和四氧化锇处理从科埃尔碘化亚铜硫代胆碱衍生的电子致密沉淀物来进行乙酰胆碱酯酶活性的超微结构定位。
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Koelle's copper thiocholine method performed with a low-pH phosphate buffer, followed by osmification of the precipitate: revival of two abandoned procedures.用低pH值磷酸盐缓冲液进行科勒硫代胆碱铜法，随后对沉淀物进行锇化处理：两种被废弃方法的复兴。
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A microfluorometric assay for cholinesterases, suitable for multiple kinetic determinations of picomoles of released thiocholine.一种用于胆碱酯酶的微量荧光测定法，适用于对皮摩尔释放硫代胆碱进行多次动力学测定。
Anal Biochem. 1983 Sep;133(2):450-6. doi: 10.1016/0003-2697(83)90107-0.
10
Cytochemical localization of cholinesterase activity at the giant synapse of the squid.乌贼巨大突触处胆碱酯酶活性的细胞化学定位。
Histochemistry. 1975 Jun 9;43(4):305-11. doi: 10.1007/BF00490189.

本文引用的文献

1
ACETYLCHOLINESTERASE IN FROG SYMPATHETIC AND DORSAL ROOT GANGLIA : A Study by Electron Microscope Cytochemistry and Microgasometric Analysis with the Magnetic Diver.蛙交感和脊根神经节中的乙酰胆碱酯酶：电子显微镜细胞化学和带有磁分离器的微量气体分析的研究
J Cell Biol. 1966 Nov 1;31(2):215-42. doi: 10.1083/jcb.31.2.215.
2
A histochemical method for localizing cholinesterase activity.一种用于定位胆碱酯酶活性的组织化学方法。
Proc Soc Exp Biol Med. 1949 Apr;70(4):617-22. doi: 10.3181/00379727-70-17013.
3
PREPARATION OF THICK SECTIONS FOR CYTOCHEMISTRY AND ELECTRON MICROSCOPY BY A NON-FREEZING TECHNIQUE.
用非冷冻技术制备用于细胞化学和电子显微镜检查的厚切片
Nature. 1963 Nov 16;200:691. doi: 10.1038/200691a0.
4
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
5
A modification of the thiocholine method for the determination of cholinesterase. I. Biochemical evaluation of selective inhibitors.一种用于测定胆碱酯酶的硫代胆碱方法的改进。I. 选择性抑制剂的生化评价
Acta Physiol Scand. 1957 Oct 22;40(4):322-30. doi: 10.1111/j.1748-1716.1957.tb01500.x.
6
Histochemical studies on acetylcholine- and choline-esterases. III. Localization in the heart of mammals.乙酰胆碱酯酶和胆碱酯酶的组织化学研究。III. 在哺乳动物心脏中的定位。
Acta Anat (Basel). 1954;22(2-3):143-51.
7
Attempts to demonstrate acetylcholinesterase activity in blood and bone-marrow cells by a modified thiocholine technique.通过改良硫代胆碱技术来证明血液和骨髓细胞中乙酰胆碱酯酶活性的尝试。
J Histochem Cytochem. 1954 Mar;2(2):115-21. doi: 10.1177/2.2.115.
8
An electron-microscopic study of cholinesterase distribution in the rat adrenal medulla.大鼠肾上腺髓质中胆碱酯酶分布的电子显微镜研究。
J Microsc. 1969;89(2):181-93. doi: 10.1111/j.1365-2818.1969.tb00664.x.
9
The appearance of acetylcholinesterase in the myotome of the embryonic rabbit. An electron microscope cytochemical and biochemical study.胚胎兔肌节中乙酰胆碱酯酶的出现。一项电子显微镜细胞化学和生物化学研究。
J Cell Biol. 1971 Dec;51(3):703-21. doi: 10.1083/jcb.51.3.703.
10
Cytochemical localization of cholinesterase activity at the giant synapse of the squid.乌贼巨大突触处胆碱酯酶活性的细胞化学定位。
Histochemistry. 1975 Jun 9;43(4):305-11. doi: 10.1007/BF00490189.