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用于胆碱酯酶组织化学显示的膜方法的可能性与局限性。

The possibilities and limitations of membrane methods for the histochemical demonstration of cholinesterases.

作者信息

Lukás Z, Fialová Z

出版信息

Histochemistry. 1976 Feb 26;46(3):245-8. doi: 10.1007/BF02462788.

DOI:10.1007/BF02462788
PMID:2569
Abstract

The thiocholine method for the histochemical detection of cholinesterases according to Karnovsky-Roots was adapted for unfixed cryostat sections by addition of the agar solution to the incubation mixture and by using the semipermeable membrane interposed between the section and the incubation medium. The procedure prevents the leakage of the enzyme activity of the section and is suitable for tissues where the cholinesterase activity is low.

摘要

根据卡诺夫斯基-鲁茨法进行胆碱酯酶组织化学检测的硫代胆碱法，通过在孵育混合物中添加琼脂溶液，并使用置于切片与孵育介质之间的半透膜，适用于未固定的低温恒温器切片。该方法可防止切片酶活性的泄漏，适用于胆碱酯酶活性较低的组织。

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引用本文的文献

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Improvement of the method of Karnovsky and Roots for the histochemical demonstration of acetylcholinesterase.

本文引用的文献

1
A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.一种用于胆碱酯酶的“直接显色”硫代胆碱法。
J Histochem Cytochem. 1964 Mar;12:219-21. doi: 10.1177/12.3.219.
2
Effect of pH on the activity of eel esterase towards different substrates.pH对鳗鱼酯酶作用于不同底物活性的影响。
Biochem J. 1958 Mar;68(3):493-9. doi: 10.1042/bj0680493.
3
Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. II. Nonspecific esterase and -glucuronidase.用于改善组织切片中酶活性组织化学显示的半透膜。II. 非特异性酯酶和β-葡萄糖醛酸酶。
Histochemistry. 1987;86(5):531-2. doi: 10.1007/BF00500628.
Histochemie. 1973 Feb 6;34(2):127-34. doi: 10.1007/BF00303985.
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[Topochemistry of beta-glycosidases in the aortae and coronary arteries of rats, guinea pigs and rabbits under normal conditions and after cholesterol feed].
Cesk Patol. 1974 Feb;10(1):1-9.
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Histochemical methods for acid beta-galactosidase: technics for semipermeable membranes.
Histochemie. 1973 Dec 31;37(4):375-8. doi: 10.1007/BF00274973.
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[Current problems in the cytochemistry of lysosomal hydrolases].[溶酶体水解酶细胞化学的当前问题]
Acta Morphol Acad Sci Hung. 1972;20(3):269-93.
7
Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. 3. Lactate dehydrogenase.用于改善组织切片中酶活性组织化学显示的半透膜。3. 乳酸脱氢酶。
Histochemie. 1973 May 22;35(2):165-72. doi: 10.1007/BF00306042.
8
Histochemical demonstration of the intestinal hetero-beta-galactosidase (glucosidase).肠道异种β-半乳糖苷酶（葡萄糖苷酶）的组织化学显示
Histochemistry. 1974;42(3):271-86. doi: 10.1007/BF00492660.
9
Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. IV. Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating).用于改进组织切片中酶活性组织化学显示的半透膜。IV. 葡萄糖6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶（脱羧）
Histochemistry. 1974;40(4):349-59. doi: 10.1007/BF00495042.
10
Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. I. Acid phosphatase.用于改善组织切片中酶活性组织化学显示的半透膜。I. 酸性磷酸酶
Histochemie. 1972;30(1):31-9. doi: 10.1007/BF00303934.