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用于胆碱酯酶定位的硫代胆碱铜组织化学方法捕获反应的定量评估。

Quantitative evaluation of the trapping reaction of copperthiocholine histochemical procedures for localization of cholinesterases.

作者信息

Grubić Z, Brzin M

出版信息

Histochemistry. 1978 Jul 12;56(3-4):213-20. doi: 10.1007/BF00495982.

DOI:10.1007/BF00495982
PMID:689917
Abstract

The aim of the present study was to investigate whether the trapping reaction of the histochemical procedure for the localization of ChE of Koelle and Friedenwald (1949) and its modification by Brzin and Pucihar (1976) proceeds quantitatively. The weight of the precipitate formed in the tissue sample during the histochemical procedure was compared with enzyme activity of an equal sample. The differential magnetic microbalance was used for measurements of reduced weight and for previous determination of density of the precipitate. The evidence for the composition of the final product was drawn from the quantitative analysis of copper and iodine and from the infrared spectra. Tsuji's statement (1974) that cuprous copper thiocholine iodide is the final product of histochemical procedures investigated was confirmed. It was found that the trapping reaction of the original as well as of the modified procedure under our experimental conditions in tissue sections proceeds quantitatively which means that one of the basic conditions for reliable localization is fulfilled.

摘要

本研究的目的是调查用于定位Koelle和Friedenwald（1949年）胆碱酯酶的组织化学方法及其由Brzin和Pucihar（1976年）所做的改进中的捕获反应是否能定量进行。将组织化学过程中组织样本中形成的沉淀物重量与等量样本的酶活性进行比较。使用差动磁微天平测量重量减轻并预先测定沉淀物的密度。最终产物组成的证据来自铜和碘的定量分析以及红外光谱。Tsuji（1974年）关于碘化亚铜硫代胆碱是所研究组织化学过程的最终产物的说法得到了证实。发现在我们的实验条件下，原始方法以及改进方法在组织切片中的捕获反应能定量进行，这意味着满足了可靠定位的基本条件之一。

相似文献

1
Quantitative evaluation of the trapping reaction of copperthiocholine histochemical procedures for localization of cholinesterases.用于胆碱酯酶定位的硫代胆碱铜组织化学方法捕获反应的定量评估。
Histochemistry. 1978 Jul 12;56(3-4):213-20. doi: 10.1007/BF00495982.
2
Ultrastructural localization of acetylcholinesterase activity by means of the electron dense precipitate derived from Koelle's cuprous thiocholine iodide by treatment with phosphomolybdic acid and osmium tetroxide.通过用磷钼酸和四氧化锇处理从科埃尔碘化亚铜硫代胆碱衍生的电子致密沉淀物来进行乙酰胆碱酯酶活性的超微结构定位。
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3
A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases.一步法与两步法硫代胆碱铜法用于胆碱酯酶细胞化学定位的比较。
Histochemistry. 1977 Feb 1;50(4):313-8. doi: 10.1007/BF00507124.
4
Iodide, thiocyanate and cyanide ions as capturing reagents in one-step copper-thiocholine method for cytochemical localization of cholinesterase activity.在一步铜硫代胆碱法中，碘离子、硫氰酸根离子和氰离子作为捕获试剂用于胆碱酯酶活性的细胞化学定位。
Histochemistry. 1976 Sep 13;48(4):283-92. doi: 10.1007/BF00499245.
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A modification of thiocholine-ferricyanide method of Karnovsky and Roots for localization of acetylcholinesterase activity without interference by Koelle's copper thiocholine iodide precipitate.卡诺夫斯基和鲁茨硫代胆碱-铁氰化物法的一种改良方法，用于乙酰胆碱酯酶活性的定位，不受科埃尔碘化硫代胆碱铜沉淀的干扰。
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Histochemical demonstration of membrane-bound acetylcholinesterase: a methodological consideration on Koelle's copper thiocholine reaction and on Karnovsky's copper ferrocyanide reaction.膜结合乙酰胆碱酯酶的组织化学显示：关于柯勒硫代胆碱铜反应和卡诺夫斯基亚铁氰化铜反应的方法学考量
Acta Histochem Suppl. 1986;33:147-56.
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Factors affecting the sensitivity and consistency of the Koelle-Friedenwald histochemical method for localization of acetylcholinesterase.影响用于乙酰胆碱酯酶定位的柯勒-弗里登瓦尔德组织化学方法敏感性和一致性的因素。
Brain Res Bull. 1984 May;12(5):543-53. doi: 10.1016/0361-9230(84)90170-9.
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Koelle's copper thiocholine method performed with a low-pH phosphate buffer, followed by osmification of the precipitate: revival of two abandoned procedures.用低pH值磷酸盐缓冲液进行科勒硫代胆碱铜法，随后对沉淀物进行锇化处理：两种被废弃方法的复兴。
Histochemistry. 1984;81(2):129-31. doi: 10.1007/BF00490105.
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A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.一种用于胆碱酯酶的“直接显色”硫代胆碱法。
J Histochem Cytochem. 1964 Mar;12:219-21. doi: 10.1177/12.3.219.
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The possibilities and limitations of membrane methods for the histochemical demonstration of cholinesterases.用于胆碱酯酶组织化学显示的膜方法的可能性与局限性。
Histochemistry. 1976 Feb 26;46(3):245-8. doi: 10.1007/BF02462788.

引用本文的文献

1
Quantitative aspects of cytochemical methods for acetylcholinesterase studied with a cytochemical model system.用细胞化学模型系统研究乙酰胆碱酯酶细胞化学方法的定量方面。
Histochemistry. 1985;83(5):443-9. doi: 10.1007/BF00509207.
2
Computerized analysis of the acetylcholinesterase distribution in the rabbit hippocampal region.兔海马区乙酰胆碱酯酶分布的计算机分析
Histochem J. 1989 Aug;21(8):481-9. doi: 10.1007/BF01845798.

本文引用的文献

1
ACETYLCHOLINESTERASE IN FROG SYMPATHETIC AND DORSAL ROOT GANGLIA : A Study by Electron Microscope Cytochemistry and Microgasometric Analysis with the Magnetic Diver.蛙交感和脊根神经节中的乙酰胆碱酯酶：电子显微镜细胞化学和带有磁分离器的微量气体分析的研究
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A histochemical method for localizing cholinesterase activity.一种用于定位胆碱酯酶活性的组织化学方法。
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Quantitative determination by a special "ampulla-diver" of cholinesterase activity in individual cells, with notes on other uses of the method.
用一种特殊的“壶腹分流器”对单个细胞中的胆碱酯酶活性进行定量测定，并介绍该方法的其他用途。
Gen Cytochem Methods. 1961;2:131-52. doi: 10.1016/b978-0-12-395584-5.50008-1.
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Quantitative evaluation of the thiocholine method for cholinesterase as applied to single end-plates from mouse gastrocnemius muscle.应用于小鼠腓肠肌单终板的硫代胆碱法测定胆碱酯酶的定量评估。
C R Trav Lab Carlsberg. 1961;32:139-53.
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On the chemistry of the thiocholine method of Koelle.关于柯勒硫代胆碱法的化学原理。
J Histochem Cytochem. 1955 Nov;3(6):441-5. doi: 10.1177/3.6.441.
6
Histochemical detection of fatal anticholinesterase poisoning.致死性抗胆碱酯酶中毒的组织化学检测
U S Armed Forces Med J. 1952 Nov;3(11):1637-44.
7
[The copper salt-thiocholine reaction used in the histochemical detection of cholinesterases].[用于胆碱酯酶组织化学检测的铜盐 - 硫代胆碱反应]
Ann Histochim. 1970 Oct-Dec;15(4):263-71.
8
A simple, rapid, and quantitative radiometric assay of acetylcholinesterase.一种简单、快速且定量的乙酰胆碱酯酶放射性测定法。
Anal Biochem. 1974 Feb;57(2):588-92. doi: 10.1016/0003-2697(74)90114-6.
9
On the chemical basis of thiocholine methods for demonstration of acetylcholinesterase activities.基于硫代胆碱法的乙酰胆碱酯酶活性检测的化学基础。
Histochemistry. 1974;42(1):99-110. doi: 10.1007/BF00498482.
10
Iodide, thiocyanate and cyanide ions as capturing reagents in one-step copper-thiocholine method for cytochemical localization of cholinesterase activity.在一步铜硫代胆碱法中，碘离子、硫氰酸根离子和氰离子作为捕获试剂用于胆碱酯酶活性的细胞化学定位。
Histochemistry. 1976 Sep 13;48(4):283-92. doi: 10.1007/BF00499245.