Steinpreis R E, Salamone J D
Department of Psychology, University of Connecticut, Storrs 06269-1020.
Brain Res. 1993 May 28;612(1-2):263-70. doi: 10.1016/0006-8993(93)91671-e.
A series of experiments were conducted in order to characterize the role of nucleus accumbens dopamine (DA) in the neurochemical and behavioral effects of phencyclidine (PCP). In the first study, microdialysis probes were implanted in nucleus accumbens to determine the effects of 4.0 and 8.0 mg/kg PCP on extracellular levels of DA and its metabolites, dihydroxyphenyl-acetic acid (DOPAC) and homovanillic acid (HVA) in behaving rats. PCP increased extracellular DA, DOPAC and HVA in the same dose range that produced increases in locomotor activity, stereotypy and ataxia. The increases in extracellular DA that were induced by 4.0 mg/kg PCP were significantly correlated with the increases in locomotor activity. In the second study, rats received bilateral injections of 6-hydroxydopamine in order to deplete DA in nucleus accumbens. DA-depleted and control rats received injections of saline and 4.0 mg/kg PCP and were tested in an 'intruder' paradigm. In this procedure, saline-and PCP-treated rats were placed in a stable colony of three other rats and social behavior was observed for 30 min. PCP reduced the frequencies of various social behaviors, but accumbens DA depletion did not reverse the effects of PCP on social behavior. Subsequently, all rats received 8.0 mg/kg PCP and were assessed for locomotor activity, stereotypy and ataxia. Depletion of DA in nucleus accumbens attenuated PCP-induced locomotion, but did not alter the effects of the drug on stereotypy or ataxia.(ABSTRACT TRUNCATED AT 250 WORDS)
为了明确伏隔核多巴胺（DA）在苯环利定（PCP）的神经化学和行为效应中的作用，进行了一系列实验。在第一项研究中，将微透析探针植入伏隔核，以确定4.0和8.0mg/kg PCP对行为大鼠细胞外DA及其代谢产物二羟基苯乙酸（DOPAC）和高香草酸（HVA）水平的影响。PCP在产生运动活动增加、刻板行为和共济失调的相同剂量范围内，增加了细胞外DA、DOPAC和HVA。4.0mg/kg PCP诱导的细胞外DA增加与运动活动增加显著相关。在第二项研究中，大鼠接受双侧6-羟基多巴胺注射，以耗尽伏隔核中的DA。DA耗尽的大鼠和对照大鼠接受盐水和4.0mg/kg PCP注射，并在“入侵者”范式中进行测试。在此过程中，将接受盐水和PCP处理的大鼠放入由另外三只大鼠组成的稳定群体中，观察30分钟的社会行为。PCP降低了各种社会行为的频率，但伏隔核DA耗尽并未逆转PCP对社会行为的影响。随后，所有大鼠接受8.0mg/kg PCP，并评估其运动活动、刻板行为和共济失调。伏隔核DA耗尽减弱了PCP诱导的运动，但未改变该药物对刻板行为或共济失调的影响。（摘要截短于250字）
