Perissel B, Benkhalifa M, Taillandier J, Dastugue N, Haus O, Charbonné F, Giraud B, Malet P
Laboratoire d'Histologie-Embryologie-Cytogénétique, Faculté de Médecine de Clermont-Ferrand, France.
Cancer Genet Cytogenet. 1993 Jun;67(2):101-7. doi: 10.1016/0165-4608(93)90160-n.
A new human malignant urologic cell line was established in vitro from a moderately differentiated transitional cell carcinoma of the bladder and cytogenetically characterized. Repeated chromosome analyses of the cell line using conventional RHG and GTG banding and non-radioactive in situ hybridization showed a stable karyotype with a modal number of 48 and chromosomal rearrangements, some of which have not been previously described. Numerical deviation included three trisomies (+7, +8, +9) and one nullisomy (-19, -19). Structural changes involved a balanced translocation (1;5)(q12;q12), an isochromosome 3q, a 14p+, and two markers. Fluorescence in situ hybridization (FISH), using biotin-labeled alpha satellite probes for chromosome 9 or painting for chromosomes 1 and 8, applied to interphase nuclei or metaphases showed similar results to those found by conventional cytogenetic study. This cell line may be an interesting model for fuller characterization by molecular biology studies and for testing anti-cancer drugs in vitro.
从膀胱中度分化移行细胞癌体外建立了一种新的人类恶性泌尿系统细胞系，并对其进行了细胞遗传学特征分析。使用传统的RHG和GTG显带以及非放射性原位杂交对该细胞系进行反复染色体分析，结果显示其核型稳定，众数为48，并存在染色体重排，其中一些重排此前未见报道。数值偏差包括三个三体（+7、+8、+9）和一个缺体（-19、-19）。结构变化涉及一个平衡易位（1；5）（q12；q12）、一条3号等臂染色体、一个14p+以及两个标记染色体。使用生物素标记的9号染色体α卫星探针或1号和8号染色体涂染探针进行的荧光原位杂交（FISH），应用于间期核或中期，结果与传统细胞遗传学研究相似。该细胞系可能是通过分子生物学研究进行更全面特征分析以及体外抗癌药物测试的一个有趣模型。
