Seruca R, Castedo S, Correia C, Gomes P, Carneiro F, Soares P, de Jong B, Sobrinho-Simões M
Unit of Genetics, Institute of Molecular Pathology and Immunology (IPATIMUP), Porto, Portugal.
Cancer Genet Cytogenet. 1993 Jul 1;68(1):42-8. doi: 10.1016/0165-4608(93)90072-t.
We describe the results of the cytogenetic study of 10 primary adenocarcinomas of the stomach and one lymph node metastasis of a gastric adenocarcinoma after direct harvesting or short-term in vitro culture. All cases showed a variable number of numerical and/or structural clonal cytogenetic aberrations. Polysomy of chromosomes 2 and 20 were the most common numerical abnormalities. Rearrangements of chromosomes 1, 3, 7, and 13 were each observed in more than half the cases. Chromosomes 3 and 13 were the chromosomes more often exhibiting structural cytogenetic aberrations. In five tumors, rearrangements of chromosome 6 resulting in partial deletion of 6q were noted (common deleted region 6q21-22-->qter). The recurrent markers observed in our series were an i(8q) and an i(17q) in three and two cases, respectively. Double minutes (dmin) or homogeneously staining regions (hsr) were evident in three tumors. Contrary to the recent claim that structural abnormalities affecting 11p13-p15 were specifically involved in gastric cancer, we detected rearrangements of this region in only two cases.
我们描述了对10例原发性胃腺癌及1例胃腺癌淋巴结转移灶进行细胞遗传学研究的结果，这些样本均是直接采集或经过短期体外培养获得的。所有病例均显示出数量不等的数值性和/或结构性克隆细胞遗传学异常。2号和20号染色体的多体性是最常见的数值异常。1号、3号、7号和13号染色体的重排在半数以上病例中均有观察到。3号和13号染色体是更常出现结构性细胞遗传学异常的染色体。在5例肿瘤中，观察到6号染色体重排导致6q部分缺失（常见缺失区域为6q21-22→qter）。在我们的系列研究中观察到的重复性标记分别为3例中的i(8q)和2例中的i(17q)。在3例肿瘤中可见双微体（dmin）或均匀染色区（hsr）。与最近声称影响11p13-p15的结构异常特别与胃癌有关的说法相反，我们仅在2例中检测到该区域的重排。
