Yang H, Binns R M
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, United Kingdom.
Cell Immunol. 1993 Jul;149(2):227-36. doi: 10.1006/cimm.1993.1150.
A polyclonal anti-porcine CD44 antibody was used to elucidate the possible role of the CD44 molecule in porcine natural cytotoxicity. It was found that the antibody inhibited porcine NK activity in a dose-dependent manner. The inhibition was reversed by incubation with soluble porcine CD44. The F(ab')2 but not Fab fragment of the antibody was effective, suggesting that the CD16 pathway was not involved and that cross-linking of the CD44 molecule might be essential for the inhibition. The antibody exerted the inhibition by acting on the effector cells, without preventing them from forming conjugates with the target cells. Kinetic studies showed that the inhibition was exerted at a late postbinding stage.
使用一种多克隆抗猪CD44抗体来阐明CD44分子在猪自然细胞毒性中的可能作用。发现该抗体以剂量依赖的方式抑制猪NK活性。用可溶性猪CD44孵育可逆转这种抑制作用。该抗体的F(ab')2片段而非Fab片段有效，这表明不涉及CD16途径，并且CD44分子的交联可能是抑制作用所必需的。该抗体通过作用于效应细胞发挥抑制作用，而不阻止它们与靶细胞形成结合物。动力学研究表明，这种抑制作用发生在结合后的晚期阶段。
