Ward R H, Lachmann P J
Immunology. 1985 Sep;56(1):179-88.
Monoclonal antibodies were raised against a human natural killing system and screened against targets lysed either by human lymphocytes in antibody-dependent cellular cytotoxicity (ADCC), or by human complement. Two monoclonals were identified which bound specifically to both types of killed targets. More detailed studies with one antibody showed that it inhibited ADCC, both as intact antibody and as the F(ab')2 fragment. Intact antibody enhanced natural (NK) killing, although the F(ab')2 fragment inhibited NK killing. The data support the hypothesis that lymphocyte-mediated killing involves a complex analogous in nature to the complement membrane attack complex. In addition, the antibodies provide evidence to suggest that this complex has antigenic determinants in common with the complement membrane attack complex, and indicate the possibility that the two systems are derived from a common ancestor.
制备了针对人类自然杀伤系统的单克隆抗体，并针对在抗体依赖性细胞毒性（ADCC）中被人类淋巴细胞裂解的靶标或被人类补体裂解的靶标进行筛选。鉴定出两种单克隆抗体，它们特异性结合两种类型的被杀伤靶标。对其中一种抗体进行的更详细研究表明，它作为完整抗体和F(ab')2片段均能抑制ADCC。完整抗体增强了自然杀伤（NK）作用，尽管F(ab')2片段抑制NK杀伤。这些数据支持这样的假说，即淋巴细胞介导的杀伤涉及一种本质上类似于补体膜攻击复合物的复合体。此外，这些抗体提供了证据表明该复合体与补体膜攻击复合物具有共同的抗原决定簇，并表明这两个系统可能源自共同祖先的可能性。
