Prabhu A, Sarin A, Saxena R K
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Cell Immunol. 1993 Jul;149(2):422-32. doi: 10.1006/cimm.1993.1167.
Soluble antigens derived from the H37Ra strain of Mycobacterium tuberculosis (Ra antigen) induced marginal proliferative response in spleen cells from unsensitized mice. The FDNB derivative of this antigen (Ra-DNP), however, had a marked proliferation-inducing effect. An increase in the population of B-cells but a reduction in the T-cell population was noted in Ra-DNP-treated spleen cell cultures. BSA-DNP derivatives used as control had no effect on mouse spleen cell proliferative activity. Moreover, addition of BSA-DNP along with Ra did not improve the proliferative response to the latter, indicating that in order to obtain proliferative activity, DNP residues must be present on Ra antigens themselves. Thymidine incorporation studies using sorted cell populations indicated that B-cells proliferated in response to Ra-DNP. The effect of Ra-DNP was neither blocked by Polymyxin-B treatment, nor retained by an endotoxin removal column, suggesting that lipopolysaccharide was not responsible for the B-cell mitogenic effect of Ra-DNP. As compared to Ra antigen, Ra-DNP derivative was found to bind more efficiently to B-cells as well as to mouse Ig. Additionally, lymph node cells derived from Ra-sensitized mice proliferated significantly better in response to Ra-DNP. These results indicate that FDNB derivatization of Ra antigen renders it more reactive with B-cells, which may in turn be responsible for a better T-cell reactivity of the derivative, since B-cells can act as antigen-presenting cells. It is possible that DNP residues may facilitate the interaction of Ra antigen with B-cells, perhaps through their Ig receptor.
来源于结核分枝杆菌H37Ra菌株的可溶性抗原（Ra抗原）可诱导未致敏小鼠脾细胞出现边缘增殖反应。然而，该抗原的2,4-二硝基氟苯衍生物（Ra-DNP）具有显著的增殖诱导作用。在经Ra-DNP处理的脾细胞培养物中，观察到B细胞数量增加而T细胞数量减少。用作对照的牛血清白蛋白-DNP衍生物对小鼠脾细胞增殖活性无影响。此外，将牛血清白蛋白-DNP与Ra一起添加并不能增强对后者的增殖反应，这表明为了获得增殖活性，DNP残基必须存在于Ra抗原自身上。使用分选细胞群体进行的胸苷掺入研究表明，B细胞对Ra-DNP有增殖反应。Ra-DNP的作用既不被多粘菌素B处理所阻断，也不被内毒素去除柱所保留，这表明脂多糖与Ra-DNP的B细胞促有丝分裂作用无关。与Ra抗原相比，发现Ra-DNP衍生物与B细胞以及小鼠Ig的结合更有效。此外，来源于经Ra致敏小鼠的淋巴结细胞对Ra-DNP的增殖反应明显更好。这些结果表明，Ra抗原的2,4-二硝基氟苯衍生化使其与B细胞的反应性更强，这反过来可能是该衍生物具有更好的T细胞反应性的原因，因为B细胞可作为抗原呈递细胞。有可能DNP残基可能通过其Ig受体促进Ra抗原与B细胞的相互作用。
