Kaye P M, Chain B M, Feldmann M
J Immunol. 1985 Mar;134(3):1930-4.
The accessory cell requirements for a given T cell response may be examined in vitro by using highly purified lymph node T cells. We have examined the capacity of different antigen-presenting cells to stimulate proliferation of Mycobacterium tuberculosis-primed T cells when the antigenic challenge is either soluble or particulate in nature. By titrations of cell number and antigen concentration, it was shown that dendritic cells are not only extremely efficient at presenting soluble mycobacterial antigen compared with various macrophage populations, but also that they are capable of presenting whole mycobacteria. Because phagocytosis of mycobacteria does not occur with these cells, we suggest that processing of antigen by dendritic cells may be initiated at the plasma membrane. Because macrophages are not essential for this in vitro response, a role for dendritic cells in antibacterial immunity in vivo is implicated.
通过使用高度纯化的淋巴结T细胞，可以在体外检测给定T细胞反应对辅助细胞的需求。当抗原刺激为可溶性或颗粒性时，我们检测了不同抗原呈递细胞刺激经结核分枝杆菌致敏的T细胞增殖的能力。通过对细胞数量和抗原浓度进行滴定，结果表明，与各种巨噬细胞群体相比，树突状细胞不仅在呈递可溶性分枝杆菌抗原方面极其高效，而且还能够呈递完整的分枝杆菌。由于这些细胞不会吞噬分枝杆菌，我们认为树突状细胞对抗原的处理可能始于质膜。由于巨噬细胞对这种体外反应并非必不可少，这暗示了树突状细胞在体内抗菌免疫中的作用。
