Malcherek P, Schultz G, Wingren U, Franzén L
Department of Pathology II, University of Linköping, Sweden.
Scand J Plast Reconstr Surg Hand Surg. 1994 Mar;28(1):1-7. doi: 10.3109/02844319409015987.
Epidermal growth factor (EGF) has been shown to stimulate connective tissue repair in the perforated mesentery of rats. The aim of the present investigation was to study the effect of EGF on the formation of healing tissue and angiogenesis in such repair. After laparotomy standardised perforations were made in the centre of the mesenteric "windows" with a scalpel. The rats were given intraperitoneal injections of either 10 micrograms EGF dissolved in phosphate-buffered saline (PBS), or PBS alone, twice daily for four consecutive days beginning on the day of operation. In the first experiment, healing tissue formation and angiogenesis was quantified morphometrically in perpendicularly cut mesenteric windows on days 1 to 10 after operation. Treatment with EGF caused the formation of significantly more healing tissue on days 2 to 7, but no stimulation of angiogenesis. In the second experiment, angiogenesis was quantified morphometrically on days 14 and 21. Mesenteric windows were spread out on objective slides after the capillary bed had been visualised by perfusion of carbon ink. Perforation caused a significant increase of microvascular density in the centre of the mesenteric windows on days 14 and 21. Treatment with EGF did not stimulate angiogenesis at any observation point. In conclusion, treatment with EGF significantly increased the formation of healing tissue in connective tissue repair in the perforated mesentery of rats, but did not affect angiogenesis.
表皮生长因子（EGF）已被证明能刺激大鼠穿孔肠系膜的结缔组织修复。本研究的目的是探讨EGF在此类修复中对愈合组织形成和血管生成的影响。剖腹术后，用手术刀在肠系膜“窗口”中心制作标准化穿孔。从手术当天开始，连续四天每天给大鼠腹腔注射两次，每次注射溶解于磷酸盐缓冲盐水（PBS）中的10微克EGF，或仅注射PBS。在第一个实验中，于术后第1至10天对垂直切割的肠系膜窗口进行形态计量学分析，以量化愈合组织形成和血管生成情况。EGF治疗在第2至7天导致形成显著更多的愈合组织，但对血管生成无刺激作用。在第二个实验中，于第14天和21天对血管生成进行形态计量学分析。在用碳墨灌注使毛细血管床可视化后，将肠系膜窗口铺展在载玻片上。穿孔导致在第14天和21天肠系膜窗口中心的微血管密度显著增加。在任何观察点，EGF治疗均未刺激血管生成。总之，EGF治疗显著增加了大鼠穿孔肠系膜结缔组织修复中愈合组织的形成，但不影响血管生成。
