Hiraoka N, Nishijima J, Murata A, Oka Y, Kitagawa K, Tanaka N, Toda H, Mori T
Department of Surgery II, Osaka University Medical School, Japan.
Eur Surg Res. 1993;25(4):222-32. doi: 10.1159/000129281.
We examined the effects of surgical trauma on polymorphonuclear leukocyte (PMN) chemotaxis, production of phagocytosis-dependent intracellular chemiluminescence (CL) and production of phagocytosis-independent total CL in two groups: group A with esophageal cancer, and group B with gastric cancer. The scale of surgical trauma was quantified by measuring interleukin-6 in plasma and exudate from drainage tubes. We found a significant augmentation of chemotaxis, total CL and intracellular CL in both groups during the post-operative week. In group A, the increments in both chemotaxis and total CL of circulating cells were smaller than those in group B, but there was no significant difference in intracellular CL between the two groups. Exudate PMNs were more chemotactic, but produced smaller amounts of total CL than circulating cells. No significant difference in intracellular CL between exudate and circulating PMNs was detected, indicating that phagocytic activity was not affected. We conclude that severe surgical trauma causes circulating PMNs with high chemotactic activity to migrate to sites of injury, but that preactivated PMNs in exudate examined in vitro produce lower amounts of reactive oxygen metabolites in response to soluble chemoattractants in vitro than cells circulating in plasma.
我们研究了手术创伤对两组患者多形核白细胞（PMN）趋化性、吞噬依赖性细胞内化学发光（CL）产生及吞噬非依赖性总CL产生的影响，这两组分别为：A组为食管癌患者，B组为胃癌患者。通过测量血浆和引流管渗出液中的白细胞介素-6来量化手术创伤的程度。我们发现两组患者在术后一周内趋化性、总CL和细胞内CL均显著增强。在A组中，循环细胞的趋化性和总CL的增加幅度小于B组，但两组细胞内CL无显著差异。渗出液中的PMN趋化性更强，但产生的总CL量比循环细胞少。未检测到渗出液和循环PMN之间细胞内CL的显著差异，表明吞噬活性未受影响。我们得出结论，严重的手术创伤会使具有高趋化活性的循环PMN迁移至损伤部位，但体外检测的渗出液中预激活的PMN对可溶性趋化剂的反应产生的活性氧代谢产物量低于血浆中循环的细胞。
