Nagayama J, Masuda Y
Laboratory of Environmental Health Sciences, School of Health Sciences, Kyushu University, Fukuoka.
Fukuoka Igaku Zasshi. 1993 May;84(5):203-11.
First, we investigated the effects of eleven methylsulphonyl polychlorinated biphenyl (MSF-PCB) homologues at 1.5 micrograms/ml and 7,8-benzoflavone (ANF) at 1.4 micrograms/ml on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 15 ng/ml)-induced aryl hydrocarbon hydroxylase (AHH) activity of cultured human lymphoblastoid cells and the following results were obtained. 1) The relative AHH activity (TCDD-induced AHH activity treated with each of the MSF-PCB compounds or ANF/the enzyme activity treated with acetone, %) of the ANF-treated culture was the smallest value (about 9%). 2) Among MSF-PCB homologues, 3-MSF-4,5,3',4'- and 4-MSF-3,5,3',4'-tetrachlorobiphenyls (TCBs) showed the highest inhibition and their relative enzyme activities were about 20%. 3) In the rest of MSF-PCB compounds, the relative AHH activities were as follows: 4-MSF-3,5,3',4',5'-pentachlorobiphenyl (PenCB); 37%, 3-MSF-4,5,2',3',4'-PenCB; 50%, 3-MSF-4,5,2',3'-TCB, 3-MSF-4,5,2',3',4',5'-hexachlorobiphenyl and 3-MSF-4,5,6,2',3',4',5'-heptachlorobiphenyl; 60 to 64%, 4-MSF-2,5,2',3',4'-PenCB; 77%, 3-MSF-2,5,3',4'-TCB; 88%, 4-MSF-2,5,2',5'-TCB; 93% and 3-MSF-4,5,3',4',5'-PenCB rather enhanced the TCDD-induced enzyme activity. Second, we prepared the hepatic microsomes of BALB/c (Ah responsive) and AKR/J (Ah nonresponsive) strains of mice after the treatment of olive oil (as control), 3-methylcholanthrene (MC, 42 mg/kg, once) and TCDD (20 micrograms/kg, 6 times, once every other week) in order to examine the effects of 3-MSF-4,5,3',4'-TCB (1.5 to 45 micrograms/ml) and ANF (1.4 to 42 micrograms/ml) on the respective hepatic microsomal AHH activities.(ABSTRACT TRUNCATED AT 250 WORDS)
首先，我们研究了浓度为1.5微克/毫升的11种甲基磺酰基多氯联苯（MSF-PCB）同系物以及浓度为1.4微克/毫升的7,8-苯并黄酮（ANF）对培养的人淋巴母细胞中2,3,7,8-四氯二苯并对二恶英（TCDD，15纳克/毫升）诱导的芳烃羟化酶（AHH）活性的影响，得到以下结果。1）ANF处理的培养物的相对AHH活性（用每种MSF-PCB化合物或ANF处理后的TCDD诱导的AHH活性/用丙酮处理后的酶活性，%）为最小值（约9%）。2）在MSF-PCB同系物中，3-MSF-4,5,3',4'-和4-MSF-3,5,3',4'-四氯联苯（TCB）显示出最高的抑制作用，其相对酶活性约为20%。3）在其余的MSF-PCB化合物中，相对AHH活性如下：4-MSF-3,5,3',4',5'-五氯联苯（PenCB）；37%，3-MSF-4,5,2',3',4'-PenCB；50%，3-MSF-4,5,2',3'-TCB、3-MSF-4,5,2',3',4',5'-六氯联苯和3-MSF-4,5,6,2',3',4',5'-七氯联苯；60%至64%，4-MSF-2,5,2',3',4'-PenCB；77%，3-MSF-2,5,3',4'-TCB；88%，4-MSF-2,5,2',5'-TCB；93%，而3-MSF-4,5,3',4',5'-PenCB反而增强了TCDD诱导的酶活性。其次，我们在给橄榄油（作为对照）、3-甲基胆蒽（MC，42毫克/千克，一次）和TCDD（20微克/千克，6次，每隔一周一次）处理后，制备了BALB/c（Ah反应型）和AKR/J（Ah无反应型）品系小鼠的肝微粒体，以研究3-MSF-4,5,3',4'-TCB（1.5至45微克/毫升）和ANF（1.4至42微克/毫升）对各自肝微粒体AHH活性的影响。（摘要截于250字）
