Kiyohara C, Mohri N, Hirohata T, Haraguchi K, Masuda Y
Department of Public Health, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Pharmacol Toxicol. 1990 Apr;66(4):273-6. doi: 10.1111/j.1600-0773.1990.tb00746.x.
The effects of 11 isomers of methylsulfonyl polychlorinated biphenyls (MSF-PCBs) on the aryl hydrocarbon hydroxylase (AHH) activity were examined at a fixed dose of 1.5 micrograms/ml by using cultured lymphoblastoid cells. One of the isomers 3-MSF-3',4,4',5-tetraCB, as well as 4-MSF-3,3',4',5-tetraCB and 4-MSF-3,3',4',5,5'-pentaCB, effectively reduced the AHH activity when added to the culture medium, especially that previously treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to induce the AHH activity. However, the inhibitory potencies exerted by the above three MSF-PCB compounds were smaller than that by 7,8-benzoflavone (BNF). When added to the culture medium simultaneously with TCDD or 3-methylcholanthrene (MC), 3-MSF-3',4,4',5-tetraCB blocked the enhancement of the AHH activity by TCDD but did not that by MC. This is in contrast to BNF which blocked the increases due to both TCDD and MC. On the other hand, 3-MSF-3',4,4',5-tetraCB added directly to the reaction mixture for the TCDD-induced AHH activity showed little influence, while BNF decreased the same activity. These results imply that the effect of 3-MSF-3',4,4',5-tetraCB on the AHH activity is different from that of BNF.
采用培养的淋巴母细胞，以1.5微克/毫升的固定剂量研究了11种甲基磺酰基多氯联苯（MSF-PCBs）异构体对芳烃羟化酶（AHH）活性的影响。异构体之一3-MSF-3',4,4',5-四氯联苯，以及4-MSF-3,3',4',5-四氯联苯和4-MSF-3,3',4',5,5'-五氯联苯，添加到培养基中时可有效降低AHH活性，尤其是先前用2,3,7,8-四氯二苯并对二恶英（TCDD）处理以诱导AHH活性的情况。然而，上述三种MSF-PCB化合物的抑制效力小于7,8-苯并黄酮（BNF）。当与TCDD或3-甲基胆蒽（MC）同时添加到培养基中时，3-MSF-3',4,4',5-四氯联苯可阻断TCDD对AHH活性的增强作用，但不能阻断MC的增强作用。这与BNF不同，BNF可阻断TCDD和MC两者引起的增加。另一方面，直接添加到TCDD诱导的AHH活性反应混合物中的3-MSF-3',4,4',5-四氯联苯影响很小，而BNF可降低相同的活性。这些结果表明3-MSF-3',4,4',5-四氯联苯对AHH活性的影响与BNF不同。
