Ragazzi E, Veronese G
J Chromatogr. 1977 Feb 1;132(1):105-14. doi: 10.1016/s0021-9673(00)93776-2.
A thin-layer chromatographic method is described in which tetracyclines are separated on a cellulose layer by development with aqueous solutions of certain salts (magnesium, calcium, barium and zinc chloride). The spots exhibit good fluorescence in UV light, allowing ready detection without the necessity for treatment with any reagents. This fluorescence can be used for the direct photometric determination of the tetracyclines with good accuracy.
本文描述了一种薄层色谱法，其中四环素在纤维素层上通过用某些盐（氯化镁、氯化钙、氯化钡和氯化锌）的水溶液展开进行分离。斑点在紫外光下呈现良好的荧光，无需用任何试剂处理即可轻松检测。这种荧光可用于直接光度法测定四环素，具有良好的准确性。
